TA321901

antibody from OriGene

Targeting: GJB3 CX31, DFNA2, EKV

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA321901

Provider

OriGene

Product name

Anti-GJB3 Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide corresponding to a region derived from 247-260 amino acids of Human gap junction protein, beta 3, 31kDa

Description

Anti-GJB3 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µl

Concentration

1.4mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted band size: 31 kDa . Positive control: Mouse brain tissue lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds)

Validation comment

WB

Immunohistochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human liver cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human liver cancer tissue using GJB3 antibody at dilution 1/35, on the right is treated with the synthetic peptide. (Original magnification:x200)

Validation comment

IHC