Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX116114 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX116114, RRID:AB_10622372
- Product name
- ARHGEF3 antibody [N1C1]
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references MicroRNA-34a modulates c-Myc transcriptional complexes to suppress malignancy in human prostate cancer cells.
MicroRNA-34a suppresses malignant transformation by targeting c-Myc transcriptional complexes in human renal cell carcinoma.
Yamamura S, Saini S, Majid S, Hirata H, Ueno K, Deng G, Dahiya R
PloS one 2012;7(1):e29722
PloS one 2012;7(1):e29722
MicroRNA-34a suppresses malignant transformation by targeting c-Myc transcriptional complexes in human renal cell carcinoma.
Yamamura S, Saini S, Majid S, Hirata H, Ueno K, Chang I, Tanaka Y, Gupta A, Dahiya R
Carcinogenesis 2012 Feb;33(2):294-300
Carcinogenesis 2012 Feb;33(2):294-300
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Sample (30 ?g of whole cell lysate) A: MCF-7 7.5% SDS PAGE GTX116114 diluted at 1:2000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- ARHGEF3 antibody [N1C1] detects ARHGEF3 protein at cytoplasm and nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: ARHGEF3 protein stained by ARHGEF3 antibody [N1C1] (GTX116114) diluted at 1:500.Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human hepatoma, using ARHGEF3(GTX116114) antibody at 1:500 dilution.