H00001175-M01

antibody from Novus Biologicals

Targeting: AP2S1 CLAPS2, FBH3, FBHOk, HHC3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001175-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00001175-M01, RRID:AB_2056492

Product name

Mouse Monoclonal AP2S1 Antibody

Antibody type

Monoclonal

Description

IgG purified. AP2S1 (3E4)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: AP2S1 Antibody (3E4) [H00001175-M01] - Analysis of AP2S1 over-expressed 293 cell line, cotransfected with AP2S1 Validated Chimera RNAi ( Cat # H00001175-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AP2S1 monoclonal antibody (M01), clone 3E4 (Cat # H00001175-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: AP2S1 Antibody (3E4) [H00001175-M01] - Analysis of AP2S1 expression in transfected 293T cell line by AP2S1 monoclonal antibody (M01), clone 3E4.Lane 1: AP2S1 transfected lysate(17 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: AP2S1 Antibody (3E4) [H00001175-M01] - Detection limit for recombinant GST tagged AP2S1 is 0.3 ng/ml as a capture antibody.