H00001175-M01

antibody from Invitrogen Antibodies

Targeting: AP2S1 CLAPS2, FBH3, FBHOk, HHC3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001175-M01

Provider

Invitrogen Antibodies

Product name

AP2S1 Monoclonal Antibody (3E4)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Sequence of this protein is as follows: MIRFILIQNR AGKTRLAKWY MQFDDDEKQK LIEEVHAVVT VRDAKHTNFV EFRNFKIIYR RYAGLYFCIC VDVNDNNLAY LEAIHNFVEV LNEYFHNVCE LDLVFNFYKV YTVVDEMFLA GEIRETSQTK VLKQLLMLQS LE

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

3E4

Vial size

100 µg

Concentration

See Label

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of AP2S1 over-expressed 293 cell line, cotransfected with AP2S1 Validated Chimera RNAi (Cat # H00001175-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with AP2S1 monoclonal antibody (M01), clone 3E4 (Cat # H00001175-M01). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of AP2S1 expression in transfected 293T cell line by AP2S1 monoclonal antibody (M01), clone 3E4.Lane 1: AP2S1 transfected lysate(17 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged AP2S1 is 0.3 ng/mL as a capture antibody.