PA5-113253

antibody from Invitrogen Antibodies

Targeting: SPPL3 DKFZP586C1324, IMP2, MGC126674, MGC126676, MGC90402, PSL4

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Antibody Data

Product number

PA5-113253

Provider

Invitrogen Antibodies

Product name

SPPL3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µg

Concentration

3 mg/mL

Storage

-20°C or -80°C if preferred

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SPPL3 using a polyclonal antibody (Product # PA5-113253) at a dilution of 2 µg/mL. A goat anti-rabbit polyclonal secondary antibody at a dilution of 1:50,000 was used for detection in Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, and MCF-7 whole cell lysate. Observed band: 43 kDa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SPPL3 in multiple sample types using a SPPL3 polyclonal antibody (Product # PA5-113253) at a dilution of 1:2,000. A goat anti rabbit polyclonal secondary antibody was used at a dilution of 1:50,000. Sample types that were tested include: Hela whole cell lysate, U87 whole cell lysate, SY5Y whole cell lysate, A549 whole cell lysate, HL-60 whole cell lysate, 293T whole cell lysate, K562 whole cell lysate, mouse brain tissue, and rat brain tissue.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SPPL3 using a polyclonal antibody (Product # PA5-113253) at a dilution of 2 µg/mL. A goat anti-rabbit polyclonal secondary antibody at a dilution of 1:50,000 was used for detection in Hela whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, and MCF-7 whole cell lysate. Observed band: 43 kDa.