LS-C801229

antibody from LSBio

Targeting: ARHGAP35 GRF-1, GRLF1, KIAA1722, P190A, p190ARhoGAP, p190RhoGAP

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C801229

Provider

LSBio

Product name

ARHGAP35 / GRLF1 Antibody (phospho-Tyr1105) LS-C801229

Antibody type

Polyclonal

Description

Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Storage

Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of Phospho-GRF-1 (Tyr1105) expression in various lysates

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Western blot analysis of GRF-1 phosphorylation expression in EGF treated 293 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

1:100 staining human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.