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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- PA5-30547 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FUBP3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: NT2D1, IMR32, U87-MG, MCF-7. Predicted reactivity: Mouse (96%), Rat (97%), Zebrafish (81%), Xenopus laevis (90%), Pig (95%), Chicken (91%), Rhesus Monkey (100%), Bovine (95%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using FUBP3 Polyclonal Antibody (Product # PA5-30547). Sample (30 µg of whole cell lysate). Lane A: MCF-7. 7.5% SDS PAGE. FUBP3 Polyclonal Antibody (Product # PA5-30547) diluted at 1:10,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of FUBP3 in paraformaldehyde-fixed MCF-7 cells using a FUBP3 polyclonal antibody (Product # PA5-30547) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of FUBP3 was performed in paraffin-embedded rat ovary tissue using FUBP3 Polyclonal Antibody (Product # PA5-30547) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of FUBP3 was performed in paraffin-embedded rat testis tissue using FUBP3 Polyclonal Antibody (Product # PA5-30547) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using FUBP3 (Product # PA5-30547) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
