LS-B10591

antibody from LSBio

Targeting: TUBA1B K-ALPHA-1

Western blot (Data presented on provider website)

ELISA (Recommended by provider)

Immunocytochemistry (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-B10591

Provider

LSBio

Product name

IHC-plus™ TUBA1B / Tubulin Alpha 1B Antibody (C-Terminus, clone 17H11.F10) LS-B10591

Antibody type

Monoclonal

Description

Protein A purified

Reactivity

Human, Mouse, Rat, Bovine, Chicken/Avian

Host

Mouse

Isotype

IgG

Antibody clone number

17H11.F10

Storage

Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Anti-alpha-Tubulin Monoclonal Antibody - Immunohistochemistry. anti-a-tubulin monoclonal antibody was used at a 2.5 ug/mL to detect tubulin in thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. tissue was formalin-fixed and paraffin embedded.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-alpha-Tubulin Monoclonal Antibody - Immunohistochemistry. anti-a-tubulin monoclonal antibody was used at a 2.5 ug/mL to detect tubulin in thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. tissue was formalin-fixed and paraffin embedded.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-TUBA1B antibody IHC staining of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Anti-alpha-Tubulin Monoclonal Antibody - Immunohistochemistry. anti-a-tubulin monoclonal antibody was used at a 2.5 ug/mL to detect tubulin in thyroid follicular epithelium (40X) showing moderate to strong cytoplasmic staining (image). Moderate to strong cytoplasmic staining was also observed within subsets of neurons and glia, and epithelial cells including adrenal, breast, colon, pancreas, kidney, prostate, placenta, skin, testis, uterus, thyroid, and within lymphoid organs. The image shows the localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain. tissue was formalin-fixed and paraffin embedded.