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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
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- Validations
- Western blot [8]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX102078 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX102078, RRID:AB_10731277
- Product name
- alpha Tubulin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Application of amniotic fluid stem cells in repairing sciatic nerve injury in minipigs.
9-bis[2-(pyrrolidin-1-yl)ethoxy]-6-{4-[2-(pyrrolidin-1-yl)ethoxy]phenyl}-11H-indeno[1, 2-c]quinolin-11-one (BPIQ), A Quinoline Derivative Inhibits Human Hepatocellular Carcinoma Cells by Inducing ER Stress and Apoptosis.
MARK2/Par1b Insufficiency Attenuates DVL Gene Transcription via Histone Deacetylation in Lumbosacral Spina Bifida.
Paradoxical overexpression of MBNL2 in hepatocellular carcinoma inhibits tumor growth and invasion.
The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels.
Thrombin promotes fibronectin secretion by bone marrow mesenchymal stem cells via the protease-activated receptor mediated signalling pathways.
Chemical proteomics identifies heterogeneous nuclear ribonucleoprotein (hnRNP) A1 as the molecular target of quercetin in its anti-cancer effects in PC-3 cells.
Su CF, Chang LH, Kao CY, Lee DC, Cho KH, Kuo LW, Chang H, Wang YH, Chiu IM
Brain research 2018 Jan 1;1678:397-406
Brain research 2018 Jan 1;1678:397-406
9-bis[2-(pyrrolidin-1-yl)ethoxy]-6-{4-[2-(pyrrolidin-1-yl)ethoxy]phenyl}-11H-indeno[1, 2-c]quinolin-11-one (BPIQ), A Quinoline Derivative Inhibits Human Hepatocellular Carcinoma Cells by Inducing ER Stress and Apoptosis.
Chang WT, Fong Y, Chuang SC, Chou CK, Chou HL, Yang CF, Tseng CH, Chen YL, Chiu CC
Anti-cancer agents in medicinal chemistry 2017;17(5):692-700
Anti-cancer agents in medicinal chemistry 2017;17(5):692-700
MARK2/Par1b Insufficiency Attenuates DVL Gene Transcription via Histone Deacetylation in Lumbosacral Spina Bifida.
Chen S, Zhang Q, Bai B, Ouyang S, Bao Y, Li H, Zhang T
Molecular neurobiology 2017 Oct;54(8):6304-6316
Molecular neurobiology 2017 Oct;54(8):6304-6316
Paradoxical overexpression of MBNL2 in hepatocellular carcinoma inhibits tumor growth and invasion.
Lee YH, Jhuang YL, Chen YL, Jeng YM, Yuan RH
Oncotarget 2016 Oct 4;7(40):65589-65601
Oncotarget 2016 Oct 4;7(40):65589-65601
The Cullin 4A/B-DDB1-Cereblon E3 Ubiquitin Ligase Complex Mediates the Degradation of CLC-1 Chloride Channels.
Chen YA, Peng YJ, Hu MC, Huang JJ, Chien YC, Wu JT, Chen TY, Tang CY
Scientific reports 2015 May 29;5:10667
Scientific reports 2015 May 29;5:10667
Thrombin promotes fibronectin secretion by bone marrow mesenchymal stem cells via the protease-activated receptor mediated signalling pathways.
Chen J, Ma Y, Wang Z, Wang H, Wang L, Xiao F, Wang H, Tan J, Guo Z
Stem cell research & therapy 2014 Mar 17;5(2):36
Stem cell research & therapy 2014 Mar 17;5(2):36
Chemical proteomics identifies heterogeneous nuclear ribonucleoprotein (hnRNP) A1 as the molecular target of quercetin in its anti-cancer effects in PC-3 cells.
Ko CC, Chen YJ, Chen CT, Liu YC, Cheng FC, Hsu KC, Chow LP
The Journal of biological chemistry 2014 Aug 8;289(32):22078-89
The Journal of biological chemistry 2014 Aug 8;289(32):22078-89
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Supportive validation
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- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: A431 B: Jurkat 10% SDS PAGE GTX102078 diluted at 1:10000
- Validation comment
- WB
- Submitted by
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- Main image
- Experimental details
- Sample (whole cell lysate) A: 293T 20ug B: 293T 10ug C: 293T 5ug D: 293T 1ug 7.5% SDS PAGE GTX102078 diluted at 1:10000
- Validation comment
- WB
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- Experimental details
- Sample (30 ug of cell lysate) A: HeLa B: HeLa cytosol fraction C: HeLa nucleus fraction 7.5% SDS PAGE GTX102078 diluted at 1:10000
- Validation comment
- WB
- Submitted by
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- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: 293T D: A431 E: HeLa F: HepG2 G: H1299 H: HCT116 I: MCF-7 J: NT2D1 K: PC-3 L: U87-MG 7.5% SDS PAGE GTX102078 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: 293T B: NIH-3T3 C: PC-12 7.5% SDS PAGE GTX102078 diluted at 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis.A. 10 μg 293T whole cell lysate/extract B. 5 μg 293T whole cell lysate/extract10 % SDS-PAGEalpha Tubulin antibody (GTX102078) dilution: 1:10000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extractB. 30 ?g HeLa whole cell lysate/extractC. 30 ?g NIH-3T3 whole cell lysate/extractD. 30 ?g PC-12 whole cell lysate/extract10 % SDS-PAGEalpha Tubulin antibody (GTX102078) dilution: 1:10000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- alpha Tubulin antibody detects alpha Tubulin protein by Western blot analysis.A. 30 μg 293T whole cell lysate/extractB. 30 μg HeLa whole cell lysate/extractC. 30 μg NIH-3T3 whole cell lysate/extractD. 30 μg PC-12 whole cell lysate/extract10 % SDS-PAGEalpha Tubulin antibody (GTX102078) dilution: 1:10000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using alpha Tubulin(GTX102078) antibody (Green) at 1:500 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded U251 xenograft, using alpha Tubulin(GTX102078) antibody at 1:500 dilution.
