Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
- Flow cytometry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- ARG65693 - Provider product page
- Provider
- Arigo
- Product name
- anti-alpha Tubulin antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide of Human alpha Tubulin protein.
- Description
- Affinity purification with immunogen.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Keep the antibody in the dark and keep protected from prolonged exposure to light. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening.
- Handling
- The antibody solution should be gently mixed before use.
No comments: Submit comment
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 30 µg of HeLa cell lysate stained with ARG65693 anti-alpha Tubulin antibody at 1:5000 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 30 µg of 1) Mouse brain, and 2) Rat brain lysates stained with ARG65693 anti-alpha Tubulin antibody at 1:500 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: HepG2 cells stained with ARG65693 anti-alpha Tubulin antibody (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunocytochemistry: HeLa cells stained with ARG65693 anti-alpha Tubulin antibody (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Human stomach tissue stained with ARG65693 anti-alpha Tubulin antibody.?
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Human tonsil tissue stained with ARG65693 anti-alpha Tubulin antibody. Counter stained with hematoxylin.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded Mouse pancreas tissue stained with ARG65693 anti-alpha Tubulin antibody. Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: HeLa cells stained with ARG65693 anti-alpha Tubulin antibody at 1:100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Secondary antibody: Goat anti-Rabbit IgG (FITC).
