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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- H00010376-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00010376-M01, RRID:AB_463841
- Product name
- TUBA1B monoclonal antibody (M01), clone 4D1
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant TUBA1B.
- Antigen sequence
MRECISIHVGQAGVQIGNACWELYCLKHGIQPDGQ
MPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDL
EPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYAR
GHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHS
FGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAP
QVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIY
DICRRNLDIERPTYTNLNRLISQIVSSITASLRFD
GALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEK
AYHEQLSVAEITNACFEPANQMVKCDPRHGKYMAC
CLLYRGDVVPKDVNAAIATIKTKRSIQFVDWCPTG
FKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAIA
EAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSE
AREDMAALEKDYEEVGVDSVEGEGEEEGEEY- Isotype
- IgG
- Antibody clone number
- 4D1
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Submitted references De novo production of K-alpha1 tubulin-specific antibodies: role in chronic lung allograft rejection.
Goers TA, Ramachandran S, Aloush A, Trulock E, Patterson GA, Mohanakumar T
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):4487-94
Journal of immunology (Baltimore, Md. : 1950) 2008 Apr 1;180(7):4487-94
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- TUBA1B monoclonal antibody (M01), clone 4D1 Western Blot analysis of TUBA1B expression in A-431 ( Cat # L015V1 ).
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of TUBA1B expression in transfected 293T cell line by TUBA1B monoclonal antibody (M01), clone 4D1.Lane 1: TUBA1B transfected lysate(50.2 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of monoclonal antibody to TUBA1B on A-431 cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to TUBA1B on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
