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- Antibody Data
- Antigen structure
- References [31]
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- Product number
- 600-401-880 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-880, RRID:AB_2137000
- Product name
- Anti-ALPHA-TUBULIN (RABBIT) Antibody - 600-401-880
- Antibody type
- Polyclonal
- Vial size
- 200 µl
Submitted references CCN2/CTGF-A Modulator of the Optic Nerve Head Astrocyte.
Absence of endogenous carnosine synthesis does not increase protein carbonylation and advanced lipoxidation end products in brain, kidney or muscle.
Structure and regulation of the nuclear exosome targeting complex guides RNA substrates to the exosome.
C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress.
PERK signaling through C/EBPδ contributes to ER stress-induced expression of immunomodulatory and tumor promoting chemokines by cancer cells.
The HIV 5' Gag Region Displays a Specific Nucleotide Bias Regulating Viral Splicing and Infectivity.
Mice deficient in the NAAG synthetase II gene Rimkla are impaired in a novel object recognition task.
Varicella-zoster virus early infection but not complete replication is required for the induction of chronic hypersensitivity in rat models of postherpetic neuralgia.
The actin regulator profilin 1 is functionally associated with the mammalian centrosome.
CRNKL1 Is a Highly Selective Regulator of Intron-Retaining HIV-1 and Cellular mRNAs.
Angiopoietin-1 Mimetic Nanoparticles for Restoring the Function of Endothelial Cells as Potential Therapeutic for Glaucoma.
The PGRMC1 Antagonist AG-205 Inhibits Synthesis of Galactosylceramide and Sulfatide.
Pathogenetic implications of early growth response 1 in Ewing sarcoma.
A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2.
Antagonism of IAPs Enhances CAR T-cell Efficacy.
The RNA exosome contributes to gene expression regulation during stem cell differentiation.
Effect of HIV-1 Tat on the formation of the mitotic spindle by interaction with ribosomal protein S3.
FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1.
Effects of sustained i.c.v. infusion of lupus CSF and autoantibodies on behavioral phenotype and neuronal calcium signaling.
The basic tilted helix bundle domain of the prolyl isomerase FKBP25 is a novel double-stranded RNA binding module.
Little Cigars are More Toxic than Cigarettes and Uniquely Change the Airway Gene and Protein Expression.
Relationships between PROMPT and gene expression.
Melanopsin-expressing ganglion cells on macaque and human retinas form two morphologically distinct populations.
Nicotinamide ameliorates palmitate-induced ER stress in hepatocytes via cAMP/PKA/CREB pathway-dependent Sirt1 upregulation.
Autoubiquitination of the 26S proteasome on Rpn13 regulates breakdown of ubiquitin conjugates.
The prolyl isomerase, FKBP25, interacts with RNA-engaged nucleolin and the pre-60S ribosomal subunit.
Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity.
FBXW7α attenuates inflammatory signalling by downregulating C/EBPδ and its target gene Tlr4.
Direct RNA sequencing mediated identification of mRNA localized in protrusions of human MDA-MB-231 metastatic breast cancer cells.
Cytoplasmic utilization of human immunodeficiency virus type 1 genomic RNA is not dependent on a nuclear interaction with gag.
Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells.
Dillinger AE, Weber GR, Mayer M, Schneider M, Göppner C, Ohlmann A, Shamonin M, Monkman GJ, Fuchshofer R
Frontiers in cell and developmental biology 2022;10:864433
Frontiers in cell and developmental biology 2022;10:864433
Absence of endogenous carnosine synthesis does not increase protein carbonylation and advanced lipoxidation end products in brain, kidney or muscle.
Wang-Eckhardt L, Becker I, Wang Y, Yuan J, Eckhardt M
Amino acids 2022 Jul;54(7):1013-1023
Amino acids 2022 Jul;54(7):1013-1023
Structure and regulation of the nuclear exosome targeting complex guides RNA substrates to the exosome.
Gerlach P, Garland W, Lingaraju M, Salerno-Kochan A, Bonneau F, Basquin J, Jensen TH, Conti E
Molecular cell 2022 Jul 7;82(13):2505-2518.e7
Molecular cell 2022 Jul 7;82(13):2505-2518.e7
C53 Interacting with UFM1-Protein Ligase 1 Regulates Microtubule Nucleation in Response to ER Stress.
Klebanovych A, Vinopal S, Dráberová E, Sládková V, Sulimenko T, Sulimenko V, Vosecká V, Macůrek L, Legido A, Dráber P
Cells 2022 Feb 5;11(3)
Cells 2022 Feb 5;11(3)
PERK signaling through C/EBPδ contributes to ER stress-induced expression of immunomodulatory and tumor promoting chemokines by cancer cells.
Sheshadri N, Poria DK, Sharan S, Hu Y, Yan C, Koparde VN, Balamurugan K, Sterneck E
Cell death & disease 2021 Nov 1;12(11):1038
Cell death & disease 2021 Nov 1;12(11):1038
The HIV 5' Gag Region Displays a Specific Nucleotide Bias Regulating Viral Splicing and Infectivity.
Grewe B, Vogt C, Horstkötter T, Tippler B, Xiao H, Müller B, Überla K, Wagner R, Asbach B, Bohne J
Viruses 2021 May 27;13(6)
Viruses 2021 May 27;13(6)
Mice deficient in the NAAG synthetase II gene Rimkla are impaired in a novel object recognition task.
Becker I, Wang-Eckhardt L, Lodder-Gadaczek J, Wang Y, Grünewald A, Eckhardt M
Journal of neurochemistry 2021 Jun;157(6):2008-2023
Journal of neurochemistry 2021 Jun;157(6):2008-2023
Varicella-zoster virus early infection but not complete replication is required for the induction of chronic hypersensitivity in rat models of postherpetic neuralgia.
Warner BE, Yee MB, Zhang M, Hornung RS, Kaufer BB, Visalli RJ, Kramer PR, Goins WF, Kinchington PR
PLoS pathogens 2021 Jul;17(7):e1009689
PLoS pathogens 2021 Jul;17(7):e1009689
The actin regulator profilin 1 is functionally associated with the mammalian centrosome.
Nejedlá M, Klebanovych A, Sulimenko V, Sulimenko T, Dráberová E, Dráber P, Karlsson R
Life science alliance 2021 Jan;4(1)
Life science alliance 2021 Jan;4(1)
CRNKL1 Is a Highly Selective Regulator of Intron-Retaining HIV-1 and Cellular mRNAs.
Xiao H, Wyler E, Milek M, Grewe B, Kirchner P, Ekici A, Silva ABOV, Jungnickl D, Full F, Thomas M, Landthaler M, Ensser A, Überla K
mBio 2021 Jan 19;12(1)
mBio 2021 Jan 19;12(1)
Angiopoietin-1 Mimetic Nanoparticles for Restoring the Function of Endothelial Cells as Potential Therapeutic for Glaucoma.
Mietzner R, Pawlak R, Tamm ER, Goepferich A, Fuchshofer R, Breunig M
Pharmaceuticals (Basel, Switzerland) 2021 Dec 24;15(1)
Pharmaceuticals (Basel, Switzerland) 2021 Dec 24;15(1)
The PGRMC1 Antagonist AG-205 Inhibits Synthesis of Galactosylceramide and Sulfatide.
Wang-Eckhardt L, Becker I, Eckhardt M
Cells 2021 Dec 13;10(12)
Cells 2021 Dec 13;10(12)
Pathogenetic implications of early growth response 1 in Ewing sarcoma.
Noh BJ, Jung WW, Kim HS, Park YK
Pathology 2019 Oct;51(6):605-609
Pathology 2019 Oct;51(6):605-609
A Functional Link between Nuclear RNA Decay and Transcriptional Control Mediated by the Polycomb Repressive Complex 2.
Garland W, Comet I, Wu M, Radzisheuskaya A, Rib L, Vitting-Seerup K, Lloret-Llinares M, Sandelin A, Helin K, Jensen TH
Cell reports 2019 Nov 12;29(7):1800-1811.e6
Cell reports 2019 Nov 12;29(7):1800-1811.e6
Antagonism of IAPs Enhances CAR T-cell Efficacy.
Michie J, Beavis PA, Freeman AJ, Vervoort SJ, Ramsbottom KM, Narasimhan V, Lelliott EJ, Lalaoui N, Ramsay RG, Johnstone RW, Silke J, Darcy PK, Voskoboinik I, Kearney CJ, Oliaro J
Cancer immunology research 2019 Feb;7(2):183-192
Cancer immunology research 2019 Feb;7(2):183-192
The RNA exosome contributes to gene expression regulation during stem cell differentiation.
Lloret-Llinares M, Karadoulama E, Chen Y, Wojenski LA, Villafano GJ, Bornholdt J, Andersson R, Core L, Sandelin A, Jensen TH
Nucleic acids research 2018 Nov 30;46(21):11502-11513
Nucleic acids research 2018 Nov 30;46(21):11502-11513
Effect of HIV-1 Tat on the formation of the mitotic spindle by interaction with ribosomal protein S3.
Kim J, Kim YS
Scientific reports 2018 Jun 6;8(1):8680
Scientific reports 2018 Jun 6;8(1):8680
FOXP3 and miR-155 cooperate to control the invasive potential of human breast cancer cells by down regulating ZEB2 independently of ZEB1.
Brown CY, Dayan S, Wong SW, Kaczmarek A, Hope CM, Pederson SM, Arnet V, Goodall GJ, Russell D, Sadlon TJ, Barry SC
Oncotarget 2018 Jun 12;9(45):27708-27727
Oncotarget 2018 Jun 12;9(45):27708-27727
Effects of sustained i.c.v. infusion of lupus CSF and autoantibodies on behavioral phenotype and neuronal calcium signaling.
Kapadia M, Bijelić D, Zhao H, Ma D, Stojanovich L, Milošević M, Andjus P, Šakić B
Acta neuropathologica communications 2017 Sep 7;5(1):70
Acta neuropathologica communications 2017 Sep 7;5(1):70
The basic tilted helix bundle domain of the prolyl isomerase FKBP25 is a novel double-stranded RNA binding module.
Dilworth D, Upadhyay SK, Bonnafous P, Edoo AB, Bourbigot S, Pesek-Jardim F, Gudavicius G, Serpa JJ, Petrotchenko EV, Borchers CH, Nelson CJ, Mackereth CD
Nucleic acids research 2017 Nov 16;45(20):11989-12004
Nucleic acids research 2017 Nov 16;45(20):11989-12004
Little Cigars are More Toxic than Cigarettes and Uniquely Change the Airway Gene and Protein Expression.
Ghosh A, Abdelwahab SH, Reeber SL, Reidel B, Marklew AJ, Garrison AJ, Lee S, Dang H, Herring AH, Glish GL, Kesimer M, Tarran R
Scientific reports 2017 Apr 27;7:46239
Scientific reports 2017 Apr 27;7:46239
Relationships between PROMPT and gene expression.
Lloret-Llinares M, Mapendano CK, Martlev LH, Lykke-Andersen S, Jensen TH
RNA biology 2016;13(1):6-14
RNA biology 2016;13(1):6-14
Melanopsin-expressing ganglion cells on macaque and human retinas form two morphologically distinct populations.
Liao HW, Ren X, Peterson BB, Marshak DW, Yau KW, Gamlin PD, Dacey DM
The Journal of comparative neurology 2016 Oct 1;524(14):2845-72
The Journal of comparative neurology 2016 Oct 1;524(14):2845-72
Nicotinamide ameliorates palmitate-induced ER stress in hepatocytes via cAMP/PKA/CREB pathway-dependent Sirt1 upregulation.
Li J, Dou X, Li S, Zhang X, Zeng Y, Song Z
Biochimica et biophysica acta 2015 Nov;1853(11 Pt A):2929-36
Biochimica et biophysica acta 2015 Nov;1853(11 Pt A):2929-36
Autoubiquitination of the 26S proteasome on Rpn13 regulates breakdown of ubiquitin conjugates.
Besche HC, Sha Z, Kukushkin NV, Peth A, Hock EM, Kim W, Gygi S, Gutierrez JA, Liao H, Dick L, Goldberg AL
The EMBO journal 2014 May 16;33(10):1159-76
The EMBO journal 2014 May 16;33(10):1159-76
The prolyl isomerase, FKBP25, interacts with RNA-engaged nucleolin and the pre-60S ribosomal subunit.
Gudavicius G, Dilworth D, Serpa JJ, Sessler N, Petrotchenko EV, Borchers CH, Nelson CJ
RNA (New York, N.Y.) 2014 Jul;20(7):1014-22
RNA (New York, N.Y.) 2014 Jul;20(7):1014-22
Regulation of asymmetric cell division and polarity by Scribble is not required for humoral immunity.
Hawkins ED, Oliaro J, Kallies A, Belz GT, Filby A, Hogan T, Haynes N, Ramsbottom KM, Van Ham V, Kinwell T, Seddon B, Davies D, Tarlinton D, Lew AM, Humbert PO, Russell SM
Nature communications 2013;4:1801
Nature communications 2013;4:1801
FBXW7α attenuates inflammatory signalling by downregulating C/EBPδ and its target gene Tlr4.
Balamurugan K, Sharan S, Klarmann KD, Zhang Y, Coppola V, Summers GH, Roger T, Morrison DK, Keller JR, Sterneck E
Nature communications 2013;4:1662
Nature communications 2013;4:1662
Direct RNA sequencing mediated identification of mRNA localized in protrusions of human MDA-MB-231 metastatic breast cancer cells.
Jakobsen KR, Sørensen E, Brøndum KK, Daugaard TF, Thomsen R, Nielsen AL
Journal of molecular signaling 2013 Sep 1;8(1):9
Journal of molecular signaling 2013 Sep 1;8(1):9
Cytoplasmic utilization of human immunodeficiency virus type 1 genomic RNA is not dependent on a nuclear interaction with gag.
Grewe B, Hoffmann B, Ohs I, Blissenbach M, Brandt S, Tippler B, Grunwald T, Uberla K
Journal of virology 2012 Mar;86(6):2990-3002
Journal of virology 2012 Mar;86(6):2990-3002
Identification of a Src tyrosine kinase/SIAH2 E3 ubiquitin ligase pathway that regulates C/EBPδ expression and contributes to transformation of breast tumor cells.
Sarkar TR, Sharan S, Wang J, Pawar SA, Cantwell CA, Johnson PF, Morrison DK, Wang JM, Sterneck E
Molecular and cellular biology 2012 Jan;32(2):320-32
Molecular and cellular biology 2012 Jan;32(2):320-32
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-Alpha Tubulin Antibody. Lane 1: whole cell lysates from mouse brain. Lane 2: rat brain. Lane 3: A431 cells. Lane 4: Jurkat cells. Lane 5: HeLa cells. Load: 35 µg per lane. Primary antibody: Alpha Tubulin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: IRDye800 rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50 kDa corresponding to alpha tubulin (arrowhead). Other band(s): none.
- Validation comment
- Western Blot
