BM753S

antibody from Acris Antibodies GmbH

Targeting: TUBA1B K-ALPHA-1

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

BM753S

Provider

Acris Antibodies GmbH

Proper citation

Acris Antibodies GmbH Cat#BM753S, RRID:AB_1008303

Product name

anti alpha Tubulin / TUBA1B (Loading Control)

Antibody type

Monoclonal

Antigen

Fraction of Tubulin purified from Pig brain by two cycles of polymerization-depolymerization

Host

Mouse

Isotype

IgG

Antibody clone number

TU-01

Vial size

0.1 mg

Concentration

1.0 mg/ml

References

Submitted references

Dazap2 modulates transcription driven by the Wnt effector TCF-4.

Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V
Nucleic acids research 2009 May;37(9):3007-20

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Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells.

Kukharskyy V, Sulimenko V, Macůrek L, Sulimenko T, Dráberová E, Dráber P
Experimental cell research 2004 Aug 1;298(1):218-28

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Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells.

Smertenko A, Blume Y, Viklický V, Opatrný Z, Dráber P
Planta 1997;201(3):349-58

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Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies.

Smertenko A, Blume Y, Viklický V, Dráber P
European journal of cell biology 1997 Feb;72(2):104-12

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gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies.

Nováková M, Dráberová E, Schürmann W, Czihak G, Viklický V, Dr-aber P
Cell motility and the cytoskeleton 1996;33(1):38-51

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Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins.

Linhartová I, Dráber P, Dráberová E, Viklický V
The Biochemical journal 1992 Dec 15;288 ( Pt 3):919-24

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Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head.

Dráber P, Dráberová E, Viklický V
Histochemistry 1991;95(5):519-24

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Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies.

Dráber P, Dráberová E, Linhartová I, Viklický V
Journal of cell science 1989 Mar;92 ( Pt 3):519-28

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Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1.

Grimm M, Breitling F, Little M
Biochimica et biophysica acta 1987 Jul 24;914(1):83-8

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Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin.

Dráber P, Dráberová E, Zicconi D, Sellitto C, Viklický V, Cappuccinelli P
European journal of cell biology 1986 Jun;41(1):82-8

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Production and characterization of a monoclonal antitubulin antibody.

Viklický V, Dráber P, Hasek J, Bártek J
Cell biology international reports 1982 Aug;6(8):725-31

Western blot

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 4. Use of anti-alpha-tubulin antibody TU-01 as a loading control (A) in an Western blotting experiment revealing the staining pattern of various cell lysates by a newly developed monoclonal antibody (B).

Immunocytochemistry

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 1. Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-Tubulin antibody (TU-01; green) and anti-Vimentin antibody (VI-01; SM3128P; red). Nucleus is stained with DAPI (blue).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 2. Immunofluorescence staining of HeLa human cervix carcinoma cell line using anti-alpha-Tubulin antibody (TU-01; red). Nucleus is stained with DAPI (blue).

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 3. Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line using anti-alpha-Tubulin antibody (TU-01; green). Nucleus is stained with DAPI (blue).

Immunoprecipitation

Supportive validation

Submitted by

Acris Antibodies GmbH (provider)

Main image

Experimental details

Figure 5. Immunoprecipitation of alpha-tubulin from HeLa and RAJI cell lysate by antibody TU-16 and its detection by antibody TU-01. IgM heavy chain (76-92 kDa) and IgM light chain (25-30 kDa) indicated. Mr of alpha tubulin is around 50 kDa.L = lysate. IPr = immunoprecipitate (reducing conditions). IPn = immunoprecipitate (non-reducing conditions).