GTX628802

antibody from GeneTex

Targeting: TUBA1B K-ALPHA-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX628802

Provider

GeneTex

Proper citation

GeneTex Cat#GTX628802, RRID:AB_2716636

Product name

alpha Tubulin antibody [GT114]

Antibody type

Monoclonal

Reactivity

Human, Mouse, Rat, Drosophila, Zebrafish

Host

Mouse

References

Submitted references

TGFβ mediates collagen production in human CRSsNP nasal mucosa-derived fibroblasts through Smad2/3-dependent pathway and CTGF induction and secretion.

Shieh JM, Tsai YJ, Chi JC, Wu WB
Journal of cellular physiology 2019 Jul;234(7):10489-10499

---

Identification of cisplatin sensitizers through high-throughput combinatorial screening.

Sun Y, Jiang W, Lu W, Song M, Liu K, Chen P, Chang A, Ling J, Chiao PJ, Hu Y, Huang P
International journal of oncology 2018 Sep;53(3):1237-1246

---

Mitochondrial Dynamics in Rat Heart Induced by 5-Fluorouracil.

Zhang D, Ma J
Medical science monitor : international medical journal of experimental and clinical research 2018 Sep 21;24:6666-6672

---

GBF1 and Arf1 interact with Miro and regulate mitochondrial positioning within cells.

Walch L, Pellier E, Leng W, Lakisic G, Gautreau A, Contremoulins V, Verbavatz JM, Jackson CL
Scientific reports 2018 Nov 20;8(1):17121

---

Generation of FHL2 homozygous knockout lines from human embryonic stem cells by CRISPR/Cas9-mediated ablation.

Chang CW, Chang CC, Hsia KC, Tsai SY
Stem cell research 2018 Mar;27:21-24

---

Visceral Adipose Tissue Immune Homeostasis Is Regulated by the Crosstalk between Adipocytes and Dendritic Cell Subsets.

Macdougall CE, Wood EG, Loschko J, Scagliotti V, Cassidy FC, Robinson ME, Feldhahn N, Castellano L, Voisin MB, Marelli-Berg F, Gaston-Massuet C, Charalambous M, Longhi MP
Cell metabolism 2018 Mar 6;27(3):588-601.e4

---

A Role for Myosin Va in Human Cytomegalovirus Nuclear Egress.

Wilkie AR, Sharma M, Pesola JM, Ericsson M, Fernandez R, Coen DM
Journal of virology 2018 Mar 15;92(6)

---

Laminar shear stress promotes mitochondrial homeostasis in endothelial cells.

Wu LH, Chang HC, Ting PC, Wang DL
Journal of cellular physiology 2018 Jun;233(6):5058-5069

---

Osteoblast-Secreted Factors Mediate Dormancy of Metastatic Prostate Cancer in the Bone via Activation of the TGFβRIII-p38MAPK-pS249/T252RB Pathway.

Yu-Lee LY, Yu G, Lee YC, Lin SC, Pan J, Pan T, Yu KJ, Liu B, Creighton CJ, Rodriguez-Canales J, Villalobos PA, Wistuba II, de Nadal E, Posas F, Gallick GE, Lin SH
Cancer research 2018 Jun 1;78(11):2911-2924

---

High speed of fork progression induces DNA replication stress and genomic instability.

Maya-Mendoza A, Moudry P, Merchut-Maya JM, Lee M, Strauss R, Bartek J
Nature 2018 Jul;559(7713):279-284

---

Development and characterization of docetaxel-loaded lecithin-stabilized micellar drug delivery system (L_sbMDDs) for improving the therapeutic efficacy and reducing systemic toxicity.

Su CY, Liu JJ, Ho YS, Huang YY, Chang VH, Liu DZ, Chen LC, Ho HO, Sheu MT
European journal of pharmaceutics and biopharmaceutics : official journal of Arbeitsgemeinschaft fur Pharmazeutische Verfahrenstechnik e.V 2018 Feb;123:9-19

---

p38 MAPK activation and H3K4 trimethylation is decreased by lactate in vitro and high intensity resistance training in human skeletal muscle.

Willkomm L, Gehlert S, Jacko D, Schiffer T, Bloch W
PloS one 2017;12(5):e0176609

---

Hsp90α Mediates BMI1 Expression in Breast Cancer Stem/Progenitor Cells through Facilitating Nuclear Translocation of c-Myc and EZH2.

Lee YC, Chang WW, Chen YY, Tsai YH, Chou YH, Tseng HC, Chen HL, Wu CC, Chang-Chien J, Lee HT, Yang HF, Wang BY
International journal of molecular sciences 2017 Sep 15;18(9)

---

Influenza A virus upregulates PRPF8 gene expression to increase virus production.

Yang CH, Li HC, Shiu YL, Ku TS, Wang CW, Tu YS, Chen HL, Wu CH, Lo SY
Archives of virology 2017 May;162(5):1223-1235

---

Improved skeletal muscle Ca²⁺ regulation in vivo following contractions in mice overexpressing PGC-1α.

Eshima H, Miura S, Senoo N, Hatakeyama K, Poole DC, Kano Y
American journal of physiology. Regulatory, integrative and comparative physiology 2017 Jun 1;312(6):R1017-R1028

---

Hijacking of the O-GlcNAcZYME complex by the HTLV-1 Tax oncoprotein facilitates viral transcription.

Groussaud D, Khair M, Tollenaere AI, Waast L, Kuo MS, Mangeney M, Martella C, Fardini Y, Coste S, Souidi M, Benit L, Pique C, Issad T
PLoS pathogens 2017 Jul;13(7):e1006518

---

Structural determinants of APOBEC3B non-catalytic domain for molecular assembly and catalytic regulation.

Xiao X, Yang H, Arutiunian V, Fang Y, Besse G, Morimoto C, Zirkle B, Chen XS
Nucleic acids research 2017 Jul 7;45(12):7494-7506

---

The Potential of Indole/Indolylquinoline Compounds in Tau Misfolding Reduction by Enhancement of HSPB1.

Chang KH, Lin CH, Chen HC, Huang HY, Chen SL, Lin TH, Ramesh C, Huang CC, Fung HC, Wu YR, Huang HJ, Lee-Chen GJ, Hsieh-Li HM, Yao CF
CNS neuroscience & therapeutics 2017 Jan;23(1):45-56

---

TGF-β1 secreted by Tregs in lymph nodes promotes breast cancer malignancy via up-regulation of IL-17RB.

Huang SC, Wei PC, Hwang-Verslues WW, Kuo WH, Jeng YM, Hu CM, Shew JY, Huang CS, Chang KJ, Lee EY, Lee WH
EMBO molecular medicine 2017 Dec;9(12):1660-1680

---

Engineered Microvasculature in PDMS Networks Using Endothelial Cells Derived from Human Induced Pluripotent Stem Cells.

Sivarapatna A, Ghaedi M, Xiao Y, Han E, Aryal B, Zhou J, Fernandez-Hernando C, Qyang Y, Hirschi KK, Niklason LE
Cell transplantation 2017 Aug;26(8):1365-1379

---

Long-term, but not short-term high-fat diet induces fiber composition changes and impaired contractile force in mouse fast-twitch skeletal muscle.

Eshima H, Tamura Y, Kakehi S, Kurebayashi N, Murayama T, Nakamura K, Kakigi R, Okada T, Sakurai T, Kawamori R, Watada H
Physiological reports 2017 Apr;5(7)

---

Induction of cyclooxygenase-2 gene by Candida albicans through EGFR, ERK, and p38 pathways in human urinary epithelium.

Wang SH, Wang SC, Chen PC, Wang ST, Liu YW
Medical mycology 2017 Apr 1;55(3):314-322

---

Vimentin Intermediate Filaments Template Microtubule Networks to Enhance Persistence in Cell Polarity and Directed Migration.

Gan Z, Ding L, Burckhardt CJ, Lowery J, Zaritsky A, Sitterley K, Mota A, Costigliola N, Starker CG, Voytas DF, Tytell J, Goldman RD, Danuser G
Cell systems 2016 Sep 28;3(3):252-263.e8

---

Regulation of CLC-1 chloride channel biosynthesis by FKBP8 and Hsp90β.

Peng YJ, Huang JJ, Wu HH, Hsieh HY, Wu CY, Chen SC, Chen TY, Tang CY
Scientific reports 2016 Sep 1;6:32444

---

Stepwise phosphorylation of p65 promotes NF-κB activation and NK cell responses during target cell recognition.

Kwon HJ, Choi GE, Ryu S, Kwon SJ, Kim SC, Booth C, Nichols KE, Kim HS
Nature communications 2016 May 25;7:11686

---

HUWE1 interacts with PCNA to alleviate replication stress.

Choe KN, Nicolae CM, Constantin D, Imamura Kawasawa Y, Delgado-Diaz MR, De S, Freire R, Smits VA, Moldovan GL
EMBO reports 2016 Jun;17(6):874-86

---

Cabozantinib is selectively cytotoxic in acute myeloid leukemia cells with FLT3-internal tandem duplication (FLT3-ITD).

Lu JW, Wang AN, Liao HA, Chen CY, Hou HA, Hu CY, Tien HF, Ou DL, Lin LI
Cancer letters 2016 Jul 1;376(2):218-25

---

EGFR and C/EBP-β oncogenic signaling is bidirectional in human glioma and varies with the C/EBP-β isoform.

Selagea L, Mishra A, Anand M, Ross J, Tucker-Burden C, Kong J, Brat DJ
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2016 Dec;30(12):4098-4108

---

Expression and regulation of 11β-hydroxysteroid dehydrogenase type 1 in first trimester human decidua cells: Implication in preeclampsia.

Funghi L, Damiani F, Yen CF, Lee CL, Lombardi A, Schatz F, Lockwood CJ, Marcolongo P, Petraglia F, Arcuri F
Molecular and cellular endocrinology 2016 Dec 5;437:163-170

---

Obatoclax, a Pan-BCL-2 Inhibitor, Targets Cyclin D1 for Degradation to Induce Antiproliferation in Human Colorectal Carcinoma Cells.

Or CR, Chang Y, Lin WC, Lee WC, Su HL, Cheung MW, Huang CP, Ho C, Chang CC
International journal of molecular sciences 2016 Dec 27;18(1)

---

Contrasting effects of stanniocalcin-related polypeptides on macrophage foam cell formation and vascular smooth muscle cell migration.

Yamamoto K, Tajima Y, Hasegawa A, Takahashi Y, Kojima M, Watanabe R, Sato K, Shichiri M, Watanabe T
Peptides 2016 Aug;82:120-127

---

miRNA-342 Regulates CEACAM1-induced Lumen Formation in a Three-dimensional Model of Mammary Gland Morphogenesis.

Weng C, Nguyen T, Shively JE
The Journal of biological chemistry 2016 Aug 5;291(32):16777-86

---

Mycobacterium tuberculosis Upregulates TNF-α Expression via TLR2/ERK Signaling and Induces MMP-1 and MMP-9 Production in Human Pleural Mesothelial Cells.

Chen WL, Sheu JR, Chen RJ, Hsiao SH, Hsiao CJ, Chou YC, Chung CL, Hsiao G
PloS one 2015;10(9):e0137979

---

Protein Phosphatase 2A in Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in Murine Lymphatic Endothelial Cells.

Chuang YF, Chen MC, Huang SW, Hsu YF, Ou G, Tsai YJ, Hsu MJ
PloS one 2015;10(8):e0137177

---

Combining paclitaxel with ABT-263 has a synergistic effect on paclitaxel resistant prostate cancer cells.

Wang C, Huang SB, Yang MC, Lin YT, Chu IH, Shen YN, Chiu YH, Hung SH, Kang L, Hong YR, Chen CH
PloS one 2015;10(3):e0120913

---

The caspase-3-p120-RasGAP module generates a NF-κB repressor in response to cellular stress.

Khalil H, Loukili N, Regamey A, Cuesta-Marban A, Santori E, Huber M, Widmann C
Journal of cell science 2015 Sep 15;128(18):3502-13

---

The effects of a novel aliphatic-chain hydroxamate derivative WMJ-S-001 in HCT116 colorectal cancer cell death.

Huang YH, Huang SW, Hsu YF, Ou G, Huang WJ, Hsu MJ
Scientific reports 2015 Oct 29;5:15900

---

Doubly Spliced RNA of Hepatitis B Virus Suppresses Viral Transcription via TATA-Binding Protein and Induces Stress Granule Assembly.

Tsai KN, Chong CL, Chou YC, Huang CC, Wang YL, Wang SW, Chen ML, Chen CH, Chang C
Journal of virology 2015 Nov;89(22):11406-19

---

Mutation of Putative N-Glycosylation Sites on Dengue Virus NS4B Decreases RNA Replication.

Naik NG, Wu HN
Journal of virology 2015 Jul;89(13):6746-60

---

Reduced secreted clusterin as a mechanism for Alzheimer-associated CLU mutations.

Bettens K, Vermeulen S, Van Cauwenberghe C, Heeman B, Asselbergh B, Robberecht C, Engelborghs S, Vandenbulcke M, Vandenberghe R, De Deyn PP, Cruts M, Van Broeckhoven C, Sleegers K
Molecular neurodegeneration 2015 Jul 16;10:30

---

Hypoxia-inducible factor 2 alpha is essential for hepatic outgrowth and functions via the regulation of leg1 transcription in the zebrafish embryo.

Lin TY, Chou CF, Chung HY, Chiang CY, Li CH, Wu JL, Lin HJ, Pai TW, Hu CH, Tzou WS
PloS one 2014;9(7):e101980

---

YC-1 inhibits proliferation of breast cancer cells by down-regulating EZH2 expression via activation of c-Cbl and ERK.

Chang LC, Lin HY, Tsai MT, Chou RH, Lee FY, Teng CM, Hsieh MT, Hung HY, Huang LJ, Yu YL, Kuo SC
British journal of pharmacology 2014 Sep;171(17):4010-25

---

Phospholipase Cδ1 induces E-cadherin expression and suppresses malignancy in colorectal cancer cells.

Satow R, Hirano T, Batori R, Nakamura T, Murayama Y, Fukami K
Proceedings of the National Academy of Sciences of the United States of America 2014 Sep 16;111(37):13505-10

---

Selective anticancer effects of a synthetic flavagline on human Oct4-expressing cancer stem-like cells via a p38 MAPK-dependent caspase-3-dependent pathway.

Emhemmed F, Ali Azouaou S, Thuaud F, Schini-Kerth V, Désaubry L, Muller CD, Fuhrmann G
Biochemical pharmacology 2014 May 15;89(2):185-96

---

Nucleic acid chaperone activity associated with the arginine-rich domain of human hepatitis B virus core protein.

Chu TH, Liou AT, Su PY, Wu HN, Shih C
Journal of virology 2014 Mar;88(5):2530-43

---

Inhibition of G9a induces DUSP4-dependent autophagic cell death in head and neck squamous cell carcinoma.

Li KC, Hua KT, Lin YS, Su CY, Ko JY, Hsiao M, Kuo ML, Tan CT
Molecular cancer 2014 Jul 15;13:172

---

Odorant receptor-mediated sperm activation in disease vector mosquitoes.

Pitts RJ, Liu C, Zhou X, Malpartida JC, Zwiebel LJ
Proceedings of the National Academy of Sciences of the United States of America 2014 Feb 18;111(7):2566-71

---

In vivo imaging of intracellular Ca2+ after muscle contractions and direct Ca2+ injection in rat skeletal muscle in diabetes.

Eshima H, Tanaka Y, Sonobe T, Inagaki T, Nakajima T, Poole DC, Kano Y
American journal of physiology. Regulatory, integrative and comparative physiology 2013 Sep 15;305(6):R610-8

---

Interferon-induced transmembrane protein 3 is a type II transmembrane protein.

Bailey CC, Kondur HR, Huang IC, Farzan M
The Journal of biological chemistry 2013 Nov 8;288(45):32184-93

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Tubulin antibody [GT114] detects TUBA1B protein by Western blot analysis.A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg K562 whole cell lysate/extract D. 30 µg THP-1 whole cell lysate/extract E. 30 µg NCI-H929 whole cell lysate/extract 10 % SDS-PAGEalpha Tubulin antibody [GT114] (GTX628802) dilution: 1:5000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Tubulin antibody [GT114] detects TUBA1B protein by Western blot analysis.A. 30 μg 293T whole cell lysate/extract B. 30 μg NIH-3T3 whole cell lysate/extract C. 30 μg mouse brain lysate/extract D. 30 μg PC-12 whole cell lysate/extract E. 30 μg rat brain lysate/extract F. 30 μg drosophila lysae/extract10 % SDS-PAGEalpha Tubulin antibody [GT114] (GTX628802) dilution: 1:5000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Tubulin antibody [GT114] detects TUBA1B protein by Western blot analysis.A. 20 µg 293T whole cell lysate/extract B. 10 µg 293T whole cell lysate/extract C. 5 µg 293T whole cell lysate/extract D. 1 µg 293T whole cell lysate/extract 10 % SDS-PAGEalpha Tubulin antibody [GT114] (GTX628802) dilution: 1:5000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Tubulin antibody [GT114] detects TUBA1B protein by Western blot analysis.A. 30 μg zebrafish eye lysate/extract10 % SDS-PAGEalpha Tubulin antibody [GT114] (GTX628802) dilution: 1:1000

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Tubulin antibody [GT114] detects alpha Tubulin protein by Western blot analysis.A. 30 µg Jurkat whole cell lysate/extract B. 30 µg Raji whole cell lysate/extract C. 30 µg 293T whole cell lysate/extract D. 30 µg A431 whole cell lysate/extract E. 30 µg HeLa whole cell lysate/extractF. 30 µg HepG2 whole cell lysate/extract G. 30 µg H1299 whole cell lysate/extract H. 30 µg HCT116 whole cell lysate/extract I. 30 µg MCF-7 whole cell lysate/extract J. 30 µg NT2D1 whole cell lysate/extract K. 30 µg PC-3 whole cell lysate/extract L. 30 µg U87-MG whole cell lysate/extract10 % SDS-PAGEalpha Tubulin antibody [GT114] (GTX628802) dilution: 1:10000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Tubulin antibody [GT114] detects alpha Tubulin protein by western blot analysis.A. 30 ?g Jurkat whole cell lysate/extractB. 30 ?g Raji whole cell lysate/extractC. 30 ?g 293T whole cell lysate/extractD. 30 ?g A431 whole cell lysate/extract E. 30 ?g HeLa whole cell lysate/extractF. 30 ?g HepG2 whole cell lysate/extractG. 30 ?g H1299 whole cell lysate/extractH. 30 ?g HCT116 whole cell lysate/extractI. 30 ?g MCF-7 whole cell lysate/extractJ. 30 ?g NT2D1 whole cell lysate/extractK. 30 ?g PC-3 whole cell lysate/extractL. 30 ?g U87-MG whole cell lysate/extract10 % SDS-PAGEalpha Tubulin antibody [GT114] (GTX628802) dilution: 1:10000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Tubulin antibody detects alpha Tubulin protein by western blot analysis. Varied amount of 293T Whole cell extracts was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody (GTX628802) at a dilution of 1:10000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:10000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

TUBA1B antibody detects TUBA1B protein at Cytoskeleton by immunofluorescent analysis. Sample: HeLa cells were fixed in -20¢J 100% methanol for 5 min.Green: TUBA1B protein stained by TUBA1B antibody (GTX628802) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoskeleton by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:1000.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Tubulin antibody [GT114] detects alpha Tubulin protein expression by immunofluorescent analysis.Sample: Cultured rat E18 primary cortical neuron, DIV 8. Cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: alpha Tubulin protein stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:250.Red: NeuN, stained by NeuN antibody (GTX132974) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human lung cancer by immunohistochemical analysis. Sample: Paraffin-embedded human lung cancer. alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in human cervix by immunohistochemical analysis. Sample: Paraffin-embedded human cervix. alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence photomicrographs of frozen sections of mouse brain.Green: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:200. The signal was developed using goat anti-rmouse IgG antibody (Dylight488) (GTX213111-04).Blue: Nuclear staining with Hoechst 33342.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

alpha Tubulin antibody [GT114] detects alpha Tubulin protein at cytoplasm in mouse colon by immunohistochemical analysis. Sample: Paraffin-embedded mouse colon.Red: alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500. Green: beta Catenin antibody [N1N2-2] (GTX101435) diluted at 1:500.Blue: Hoechst 33342 staining.