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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
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- Product number
- NB100-55319 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-55319, RRID:AB_2227077
- Product name
- Rabbit Polyclonal Apc5 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Proteasome-dependent disruption of the E3 ubiquitin ligase anaphase-promoting complex by HCMV protein pUL21a.
Inactivation and disassembly of the anaphase-promoting complex during human cytomegalovirus infection is associated with degradation of the APC5 and APC4 subunits and does not require UL97-mediated phosphorylation of Cdh1.
Fehr AR, Gualberto NC, Savaryn JP, Terhune SS, Yu D
PLoS pathogens 2012;8(7):e1002789
PLoS pathogens 2012;8(7):e1002789
Inactivation and disassembly of the anaphase-promoting complex during human cytomegalovirus infection is associated with degradation of the APC5 and APC4 subunits and does not require UL97-mediated phosphorylation of Cdh1.
Tran K, Kamil JP, Coen DM, Spector DH
Journal of virology 2010 Oct;84(20):10832-43
Journal of virology 2010 Oct;84(20):10832-43
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Apc5 Antibody [NB100-55319] - Detection of Human and Mouse APC5 on HeLa whole cell lysates using A301-026A. APC5 was also IPed by A301-025A and BL4443. For blotting IPed APC5, ReliaBLOTTM Reagents and Procedures WB120 were used.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Apc5 Antibody [NB100-55319] - Detection of mouse APC5 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-APC5 antibody NB100-55319 used for WB at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Apc5 Antibody [NB100-55319] - Detection of human APC5 by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-APC5 antibody NB100-55319 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Apc5 Antibody [NB100-55319] - Detection of human APC5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-APC5 antibody NB100-55319 (lot NB100-55319-2) used for IP at 3 ug per reaction. APC5 was also immunoprecipitated by a previous lot of this antibody (lot NB100-55319-1) and another rabbit anti-APC5 antibody. Detection: Chemiluminescence with an exposure time of 3 minutes.
