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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- H00084678-M09 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00084678-M09, RRID:AB_538764
- Product name
- Mouse Monoclonal KDM2B Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. FBXL10 - F-box and leucine-rich repeat protein 10
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Remodeling and destabilization of chromosome 1 pericentromeric heterochromatin by SSX proteins.
KDM2A represses transcription of centromeric satellite repeats and maintains the heterochromatic state.
JHDM1B/FBXL10 is a nucleolar protein that represses transcription of ribosomal RNA genes.
Traynor S, Møllegaard NE, Jørgensen MG, Brückmann NH, Pedersen CB, Terp MG, Johansen S, Dejardin J, Ditzel HJ, Gjerstorff MF
Nucleic acids research 2019 Jul 26;47(13):6668-6684
Nucleic acids research 2019 Jul 26;47(13):6668-6684
KDM2A represses transcription of centromeric satellite repeats and maintains the heterochromatic state.
Frescas D, Guardavaccaro D, Kuchay SM, Kato H, Poleshko A, Basrur V, Elenitoba-Johnson KS, Katz RA, Pagano M
Cell cycle (Georgetown, Tex.) 2008 Nov 15;7(22):3539-47
Cell cycle (Georgetown, Tex.) 2008 Nov 15;7(22):3539-47
JHDM1B/FBXL10 is a nucleolar protein that represses transcription of ribosomal RNA genes.
Frescas D, Guardavaccaro D, Bassermann F, Koyama-Nasu R, Pagano M
Nature 2007 Nov 8;450(7167):309-13
Nature 2007 Nov 8;450(7167):309-13
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KDM2B Antibody (5G1) [H00084678-M09] - Western Blot analysis of FBXL10 expression in Hela NE ( Cat # L013V3 ).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: KDM2B Antibody (5G1) [H00084678-M09] - Detection limit for recombinant GST tagged FBXL10 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: KDM2B Antibody (5G1) [H00084678-M09] - Analysis of monoclonal antibody to FBXL10 on HeLa cell. Antibody concentration 10 ug/ml
