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- Antigen structure
- References [10]
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- Western blot [2]
- Flow cytometry [1]
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- Product number
- NB120-6125 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB120-6125, RRID:AB_2236419
- Product name
- Mouse Monoclonal PABP Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified.
- Reactivity
- Human, Mouse, Bovine, Canine, Drosophila, Simian, Xenopus
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references EJC core component MLN51 interacts with eIF3 and activates translation.
Direct stimulation of translation by the multifunctional herpesvirus ICP27 protein.
The ARE-associated factor AUF1 binds poly(A) in vitro in competition with PABP.
miRNP:mRNA association in polyribosomes in a human neuronal cell line.
miRNP:mRNA association in polyribosomes in a human neuronal cell line.
Human PABP binds AU-rich RNA via RNA-binding domains 3 and 4.
Human PABP binds AU-rich RNA via RNA-binding domains 3 and 4.
PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays.
A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex.
A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex.
Chazal PE, Daguenet E, Wendling C, Ulryck N, Tomasetto C, Sargueil B, Le Hir H
Proceedings of the National Academy of Sciences of the United States of America 2013 Apr 9;110(15):5903-8
Proceedings of the National Academy of Sciences of the United States of America 2013 Apr 9;110(15):5903-8
Direct stimulation of translation by the multifunctional herpesvirus ICP27 protein.
Larralde O, Smith RW, Wilkie GS, Malik P, Gray NK, Clements JB
Journal of virology 2006 Feb;80(3):1588-91
Journal of virology 2006 Feb;80(3):1588-91
The ARE-associated factor AUF1 binds poly(A) in vitro in competition with PABP.
Sagliocco F, Laloo B, Cosson B, Laborde L, Castroviejo M, Rosenbaum J, Ripoche J, Grosset C
The Biochemical journal 2006 Dec 1;400(2):337-47
The Biochemical journal 2006 Dec 1;400(2):337-47
miRNP:mRNA association in polyribosomes in a human neuronal cell line.
Nelson PT, Hatzigeorgiou AG, Mourelatos Z
RNA (New York, N.Y.) 2004 Mar;10(3):387-94
RNA (New York, N.Y.) 2004 Mar;10(3):387-94
miRNP:mRNA association in polyribosomes in a human neuronal cell line.
Nelson PT, Hatzigeorgiou AG, Mourelatos Z
RNA (New York, N.Y.) 2004 Mar;10(3):387-94
RNA (New York, N.Y.) 2004 Mar;10(3):387-94
Human PABP binds AU-rich RNA via RNA-binding domains 3 and 4.
Sladic RT, Lagnado CA, Bagley CJ, Goodall GJ
European journal of biochemistry 2004 Jan;271(2):450-7
European journal of biochemistry 2004 Jan;271(2):450-7
Human PABP binds AU-rich RNA via RNA-binding domains 3 and 4.
Sladic RT, Lagnado CA, Bagley CJ, Goodall GJ
European journal of biochemistry 2004 Jan;271(2):450-7
European journal of biochemistry 2004 Jan;271(2):450-7
PABP1 identified as an arginine methyltransferase substrate using high-density protein arrays.
Lee J, Bedford MT
EMBO reports 2002 Mar;3(3):268-73
EMBO reports 2002 Mar;3(3):268-73
A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex.
Grosset C, Chen CY, Xu N, Sonenberg N, Jacquemin-Sablon H, Shyu AB
Cell 2000 Sep 29;103(1):29-40
Cell 2000 Sep 29;103(1):29-40
A mechanism for translationally coupled mRNA turnover: interaction between the poly(A) tail and a c-fos RNA coding determinant via a protein complex.
Grosset C, Chen CY, Xu N, Sonenberg N, Jacquemin-Sablon H, Shyu AB
Cell 2000 Sep 29;103(1):29-40
Cell 2000 Sep 29;103(1):29-40
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PABP Antibody (10E10) [NB120-6125] - Cell line lysates were separated on SDS-PAGE and probed with 1 ug/mL Monoclonal Anti-PABP Clone: 10E10. The antibody was developed using Goat Anti-Mouse IgG-Peroxidase and a chemiluminescent substrate. (1) HEK293T, (2) HeLa, (3) G361 and (4) COS-7.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PABP Antibody (10E10) [NB120-6125] - Analysis of PABP expression in 1) A-431, 2) HeLa and 3) NIH-3T3 whole cell lysates.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: PABP Antibody (10E10) [NB120-6125] - An intracellular stain was performed on A431 cells with PABP [10E10] Antibody NB120-6125AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
