- PA5-49403 - Invitrogen Antibodies EHD2 antibody

Submitted references Hypoxia-induced macropinocytosis represents a metabolic route for liver cancer.

Zhang MS, Cui JD, Lee D, Yuen VW, Chiu DK, Goh CC, Cheu JW, Tse AP, Bao MH, Wong BPY, Chen CY, Wong CM, Ng IO, Wong CC
Nature communications 2022 Feb 17;13(1):954

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of EHD2 in lysates from A549, Hela cell line, human placenta, mouse lung, rat lung tissue (from left to right). Samples were probed with EHD2 Antibody (C-term) (Product # PA5-49403) diluted at 1:1000. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of EHD2 in paraffin-embedded human heart tissue. Sample was probed using EHD2 Antibody (C-term) (Product # PA5-49403)at a 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemical analysis of EHD2 in paraffin-embedded human placenta tissue section. Samples were probed with a EHD2 Antibody at a 1:25 dilution (Product # PA5-49403) followed by undiluted biotinylated goat polyvalent secondary antibody and DAB staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6 EHD2 promotes HCC growth and macropinocytosis in vivo. a Left: 6-8-week-old male nude mice were orthotopically implanted with luciferase-labeled MHCC97L-EV, -EHD2-KO#4, and -EHD2-KO#13 cells into the livers. HCCs were harvested 6 weeks after implantation. Middle: Livers (HCCs) harvested from the tumor-bearing mice (top) and bioluminescent imaging of lung metastases (bottom). Right: Confocal images demonstrate ex vivo dextran uptake/macropinosomes (red) and nuclei (DAPI, blue) in HCCs (top). Tumor volumes, bioluminescence of lung metastases, and macropinocytic indexes of HCC tissues were quantified (bottom). n = 5 biologically independent samples. b Left: HDTVi was performed in 8-10-week-old male wild-type C57BL/6 N mice to induce Ehd2 EV ; Tp53 KO ; c-Myc OE (EV) and Ehd2 KO ; Tp53 KO ; c-Myc OE (KO) HCCs. Middle: Image of livers (HCCs) was shown (top). Western blots demonstrate EHD2 protein expressions in EV and KO HCC tissues (bottom). Right: Confocal images show ex vivo dextran uptake/macropinosomes (red) and nuclei (DAPI, blue) in HCCs (top). Liver weights and macropinocytic indexes of HCCs (bottom). n = 5 biologically independent samples. c Confocal images show dextran uptake/macropinosomes (red) and nuclei (DAPI, blue) in orthotopic HCC tissues in nude mice. In vivo dextran labeling was performed by IV injection. n = 5 biologically independent samples. d Confocal images show dextran uptake/macropinosomes (red) and nuclei (DAPI, blue) in HCC tissues in HDTVi model

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 7 HIF-1/EHD2 induces macropinocytosis to support HCC growth in hypoxic conditions. a Transgenic strategy for generation of C57BL/6 N EHD2 germline KO ( Ehd2 -/- ) mouse. Exon3 of mouse Ehd2 was spliced in Ehd2 -/- mice. b Left: Gel picture shows PCR products from WT ( Ehd2 +/+ ) and KO ( Ehd2 -/- ) alleles in Ehd2 +/+ , Ehd2 -/- , and Ehd2 +/- mice. Right: Western blots demonstrate EHD2 expression in liver tissues from 8-week-old Ehd2 +/+ , Ehd2 +/- , and Ehd2 -/- male mice. Results were representative for three independent experiments with similar results. c Top: Image of livers ( Tp53 KO ; c-Myc OE HCCs) from Ehd2 +/+ and Ehd2 -/- mice. Middle: Confocal images show ex vivo dextran uptake/macropinosomes (red) and nuclei (DAPI, blue) in Ehd2 +/+ and Ehd2 -/- HCC tissues. Bottom: Liver weights and macropinocytic indexes from Ehd2 +/+ and Ehd2 -/- mice. n = 6 biologically independent samples. d Top: Image of livers ( Keap1 KO ; c-Myc OE HCCs) from Ehd2 +/+ and Ehd2 -/- mice. Middle: Confocal images show ex vivo dextran uptake/macropinosomes (red) and nuclei (DAPI, blue). Bottom: Liver weights and macropinocytic indexes from Ehd2 +/+ and Ehd2 -/- mice. n = 5 biologically independent samples. e Top: Image of livers (DEN/CCl 4 HCCs) was shown. Middle: Confocal images demonstrate ex vivo dextran uptake/macropinosomes (red) and nuclei (DAPI, blue) in HCC tissues from Ehd2 +/+ and Ehd2 -/- mice. Bottom: Liver weights and macropinocytic indexes from Ehd2 +/+ and Ehd2 -/- mice. n

PA5-49403

Antibody data