17004-1-AP

antibody from Invitrogen Antibodies

Targeting: HPD 4-HPPD, 4HPPD, GLOD3, PPD

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

17004-1-AP

Provider

Invitrogen Antibodies

Product name

HPD Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MTTYSDKGA KPERGRFLHF HSVTFWVGNA KQAASFYCSK MGFEPLAYRG LETGSREVVS HVIKQGKIVF VLSSALNPWN KEMGDHLVKH GDGVKDIAFE VEDCDYIVQK ARERGAKIMR EPWVEQDKFG KVKFAVLQTY GDTTHTLVEK MNYIGQFLPG YEAPAFMDPL LPKLPKCSLE MIDHIVGNQP DQEMVSASEW YLKNLQFHRF WSVDDTQVHT EYSSLRSIVV ANYEESIKMP INEPAPGKKK SQIQEYVDYN GGAGVQHIAL KTEDIITAIR HLRERGLEFL SVPSTYYKQL REKLKTAKIK VKENIDALEE LKILVDYDEK GYLLQIFTKP VQDRPTLFLE VIQRHNHQGF GAGNFNSLFK AFEEEQNLRG NLTNMETNGV VPGM (1-393 aa encoded by BC024287)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.13 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse liver tissue were subjected to SDS PAGE followed by western blot with 17004-1-AP (HPD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 17004-1-AP (HPD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HepG2 cells were subjected to SDS PAGE followed by western blot with 17004-1-AP (HPD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Various lysates were subjected to SDS PAGE followed by western blot with 17004-1-AP (HPD antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 17004-1-AP (HPD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed HepG2 cells using 17004-1-AP (HPD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 17004-1-AP (HPD antibody) at dilution of 1:100 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human liver using 17004-1-AP (HPD antibody) at dilution of 1:100 (under 40x lens).