F54982

antibody from NSJ Bioreagents

Targeting: HPD 4-HPPD, 4HPPD, GLOD3, PPD

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

F54982

Provider

NSJ Bioreagents

Product name

4-hydroxyphenylpyruvate dioxygenase Antibody / 4HPPD / HPD

Antibody type

Polyclonal

Description

Purified

Reactivity

Human, Mouse

Host

Rabbit

Vial size

0.08 µl, 0.4 µl

Concentration

In 1X PBS, pH 7.4, with 0.09% sodium azide

Storage

Aliquot the HPD antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of mouse liver tissue lysate with HPD antibody. Predicted molecular weight ~45 kDa.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of human HepG2 cell lysate with HPD antibody. Predicted molecular weight ~45 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC testing of FFPE human hepatocellular carcinoma tissue with HPD antibody. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human HepG2 cells with HPD antibody; Blue=isotype control, Green= HPD antibody.