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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PAB1749 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1749, RRID:AB_1238123
- Product name
- SMURF2 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of SMURF2.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Regulation of Smad degradation and activity by Smurf2, an E3 ubiquitin ligase.
Smurf2 is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling.
Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF beta receptor for degradation.
Zhang Y, Chang C, Gehling DJ, Hemmati-Brivanlou A, Derynck R
Proceedings of the National Academy of Sciences of the United States of America 2001 Jan 30;98(3):974-9
Proceedings of the National Academy of Sciences of the United States of America 2001 Jan 30;98(3):974-9
Smurf2 is a ubiquitin E3 ligase mediating proteasome-dependent degradation of Smad2 in transforming growth factor-beta signaling.
Lin X, Liang M, Feng XH
The Journal of biological chemistry 2000 Nov 24;275(47):36818-22
The Journal of biological chemistry 2000 Nov 24;275(47):36818-22
Smad7 binds to Smurf2 to form an E3 ubiquitin ligase that targets the TGF beta receptor for degradation.
Kavsak P, Rasmussen RK, Causing CG, Bonni S, Zhu H, Thomsen GH, Wrana JL
Molecular cell 2000 Dec;6(6):1365-75
Molecular cell 2000 Dec;6(6):1365-75
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- The SMURF2 polyclonal antibody (Cat # PAB1749) is used in Western blot to detect SMURF2 in mouse brain tissue lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue reacted with SMURF2 polyclonal antibody (Cat # PAB1749) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
