Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- NB100-2817 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2817, RRID:AB_2096172
- Product name
- Goat Polyclonal AGO1/EIF2C1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This product is not expected to cross-react with EIF2C2, EIF2C3 and EIF2C4.
- Reactivity
- Human
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Polycomb YY1 is a critical interface between epigenetic code and miRNA machinery after exposure to hypoxia in malignancy.
Functional importance of Dicer protein in the adaptive cellular response to hypoxia.
Human RISC couples microRNA biogenesis and posttranscriptional gene silencing.
Infante T, Mancini FP, Lanza A, Soricelli A, de Nigris F, Napoli C
Biochimica et biophysica acta 2015 May;1853(5):975-86
Biochimica et biophysica acta 2015 May;1853(5):975-86
Functional importance of Dicer protein in the adaptive cellular response to hypoxia.
Ho JJ, Metcalf JL, Yan MS, Turgeon PJ, Wang JJ, Chalsev M, Petruzziello-Pellegrini TN, Tsui AK, He JZ, Dhamko H, Man HS, Robb GB, Teh BT, Ohh M, Marsden PA
The Journal of biological chemistry 2012 Aug 17;287(34):29003-20
The Journal of biological chemistry 2012 Aug 17;287(34):29003-20
Human RISC couples microRNA biogenesis and posttranscriptional gene silencing.
Gregory RI, Chendrimada TP, Cooch N, Shiekhattar R
Cell 2005 Nov 18;123(4):631-40
Cell 2005 Nov 18;123(4):631-40
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AGO1/EIF2C1 Antibody [NB100-2817] - HEK293 overexpressing AGO1/EIF2C1 and probed with NB100-2817 (non-transfected HEK293 in lane B).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Small Intestine. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: AGO1/EIF2C1 Antibody [NB100-2817] - Staining of paraffin embedded Human Placenta. Antibody at 2.5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.