GTX111525

antibody from GeneTex

Targeting: HK2

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX111525

Provider

GeneTex

Proper citation

GeneTex Cat#GTX111525, RRID:AB_10724113

Product name

Hexokinase II antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse, Rat

Host

Rabbit

References

Submitted references

Adenine Nucleotide Translocase 2 as an Enzyme Related to [¹⁸F] FDG Accumulation in Various Cancers.

Lee CH, Kim MJ, Lee HH, Paeng JC, Park YJ, Oh SW, Chai YJ, Kim YA, Cheon GJ, Kang KW, Youn H, Chung JK
Molecular imaging and biology : MIB : the official publication of the Academy of Molecular Imaging 2018 Sep 17;

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PIM2-mediated phosphorylation of hexokinase 2 is critical for tumor growth and paclitaxel resistance in breast cancer.

Yang T, Ren C, Qiao P, Han X, Wang L, Lv S, Sun Y, Liu Z, Du Y, Yu Z
Oncogene 2018 Nov;37(45):5997-6009

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Proteomic analysis of oral cancer reveals new potential therapeutic targets involved in the Warburg effect.

Huang YP, Chang NW
Clinical and experimental pharmacology & physiology 2017 Aug;44(8):880-887

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (30 ?g of whole cell lysate) A: IMR32 7.5% SDS PAGE GTX111525 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample (20 ?g of whole cell lysate) A: mouse brain 7.5% SDS PAGE GTX111525 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody (GTX111525) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Hexokinase II antibody (GTX111525) diluted at 1:10000.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Hexokinase II antibody detects HK2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: HK2 protein stained by Hexokinase II antibody (GTX111525) diluted at 1:500.Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Hexokinase II antibody detects Hexokinase II protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Hexokinase II protein stained by Hexokinase II antibody (GTX111525) diluted at 1:500.Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Hexokinase II antibody detects HK2 protein at cytosol on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Hexokinase II antibody (GTX111525) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Hexokinase II antibody detects Hexokinase II protein at cytosol on mouse muscle by immunohistochemical analysis. Sample: Paraffin-embedded mouse muscle. Hexokinase II antibody (GTX111525) dilution: 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat kidney by immunohistochemical analysis. Sample: Paraffin-embedded rat kidney. Hexokinase II antibody (GTX111525) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat heart by immunohistochemical analysis. Sample: Paraffin-embedded rat heart. Hexokinase II antibody (GTX111525) diluted at 1:500.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Hexokinase II antibody detects Hexokinase II protein at cytoplasm in rat stomach by immunohistochemical analysis. Sample: Paraffin-embedded rat stomach. Hexokinase II antibody (GTX111525) diluted at 1:500.