Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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Validation data
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- Product number
- H00009183-M05 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00009183-M05, RRID:AB_2221056
- Product name
- Mouse Monoclonal ZW10 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. ZW10 (2E5)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZW10 Antibody (2E5) [H00009183-M05] - Western blot analysis of ZW10 over-expressed 293 cell line, cotransfected with ZW10 Validated Chimera RNAi or non-transfected control. Blot probed with H00009183-M05. GAPDH (36.1 kDa) used as loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZW10 Antibody (2E5) [H00009183-M05] - Analysis of ZW10 expression in transfected 293T cell line by ZW10 monoclonal antibody (M05), clone 2E5. Lane 1: ZW10 transfected lysatE (88.8 KDa). Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: ZW10 Antibody (2E5) [H00009183-M05] - Detection limit for recombinant GST tagged ZW10 is approximately 0.1ng/ml as a capture antibody.