12271-1-AP

antibody from Invitrogen Antibodies

Targeting: UCK1 FLJ12255, URK1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

12271-1-AP

Provider

Invitrogen Antibodies

Product name

UCK1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MASAGGEDC ESPAPEADRP HQRPFLIGVS GGTASGKSTV CEKIMELLGQ NEVEQRQRKV VILSQDRFYK VLTAEQKAKA LKGQYNFDHP DAFDNDLMHR TLKNIVEGKT VEVPTYDFVT HSRLPETTVV YPADVVLFEG ILVFYSQEIR DMFHLRLFVD TDSDVRLSRR DKEVCRCDHP TRSGQYGCHQ PDRAAHPGHS EW (1-201 aa encoded by BC015547)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.24 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MCF7 cells were subjected to SDS PAGE followed by western blot with 12271-1-AP (UCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of MCF-7 cells using 12271-1-AP (UCK1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-UCK1 (IP:12271-1-AP, 4ug; Detection:12271-1-AP 1:1000) with MCF-7 cells lysate 2800ug.