H00001406-M01

antibody from Abnova Corporation

Targeting: CRX CORD2, CRD, LCA7, OTX3

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Antibody Data

Product number

H00001406-M01

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00001406-M01, RRID:AB_425381

Product name

CRX monoclonal antibody (M01), clone F6-C2

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a full length recombinant CRX.

Antigen sequence

MMAYMNPGPHYSVNALALSGPSVDLMHQAVPYPSA
PRKQRRERTTFTRSQLEELEALFAKTQYPDVYARE
EVALKINLPESRVQVWFKNRRAKCRQQRQQQKQQQ
QPPGGQAKARPAKRKAGTSPRPSTDVCPDPLGISD
SYSPPLPGPSGSPTTAVATVSIWSPASESPLPEAQ
RAGLVASGPSLTSAPYAMTYAPASAFCSSPSAYGS
PSSYFSGLDPYLSPMVPQLGGPALSPLSGPSVGPS
LAQSPTSLSGQSYGAYSPVDSLEFKDPTGTWKFTY
NPMDPLDYKDQSAWKFQIL


Isotype

IgG

Antibody clone number

F6-C2

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Effects of extracellular matrix and neighboring cells on induction of human embryonic stem cells into retinal or retinal pigment epithelial progenitors.

Gong J, Sagiv O, Cai H, Tsang SH, Del Priore LV
Experimental eye research 2008 Jun;86(6):957-65

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of CRX expression in transfected 293T cell line by CRX monoclonal antibody (M01), clone F6-C2.Lane 1: CRX transfected lysate(32.261 KDa).Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged CRX is approximately 1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol