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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- ELISA [1]
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- Product number
- H00001406-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00001406-M01, RRID:AB_538008
- Product name
- Mouse Monoclonal CRX/CORD2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. CRX - cone-rod homeobox
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Effects of extracellular matrix and neighboring cells on induction of human embryonic stem cells into retinal or retinal pigment epithelial progenitors.
Gong J, Sagiv O, Cai H, Tsang SH, Del Priore LV
Experimental eye research 2008 Jun;86(6):957-65
Experimental eye research 2008 Jun;86(6):957-65
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRX/CORD2 Antibody (F6-C2) [H00001406-M01] - Analysis of CRX over-expressed 293 cell line, cotransfected with CRX Validated Chimera RNAi ( Cat # H00001406-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with CRX monoclonal antibody (M01), clone F6-C2 (Cat # H00001406-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: CRX/CORD2 Antibody (F6-C2) [H00001406-M01] - Analysis of CRX expression in transfected 293T cell line by CRX monoclonal antibody (M01), clone F6-C2.Lane 1: CRX transfected lysate(32.261 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: CRX/CORD2 Antibody (F6-C2) [H00001406-M01] - Detection limit for recombinant GST tagged CRX is approximately 1ng/ml as a capture antibody.
