MA5-17151

antibody from Invitrogen Antibodies

Targeting: PIWIL4 FLJ36156, HIWI2, Miwi2

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-17151

Provider

Invitrogen Antibodies

Product name

PIWIL4 Monoclonal Antibody (10G9B11)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-17151 targets PIWIL4 in indirect ELISA, FACS, IHC and WB applications and shows reactivity with Human samples. The MA5-17151 immunogen is purified recombinant fragment of human PIWIL4 (amino acids: 304-434) expressed in E. Coli. MA5-17151 detects PIWIL4 which has a predicted molecular weight of approximately 96.6kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

10G9B11

Vial size

100 µg

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Expression and Regulation of PIWIL-Proteins and PIWI-Interacting RNAs in Rheumatoid Arthritis.

Pleštilová L, Neidhart M, Russo G, Frank-Bertoncelj M, Ospelt C, Ciurea A, Kolling C, Gay RE, Michel BA, Vencovský J, Gay S, Jüngel A
PloS one 2016;11(11):e0166920

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PIWIL4 using a PIWIL4 monoclonal antibody (Product # MA5-17151) against a human PIWIL4 recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PIWIL4 in PC-3 (lane 1) and PANC-1 (lane 2) cell lysate using PIWIL4 monoclonal antibody (Product # MA5-17151).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PIWIL4 using PIWIL4 monoclonal antibody (Product # MA5-17151) in HEK293 (1) and PIWIL4 (AA: 304-434) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PIWIL4 in PC-3 (lane 1) and PANC-1 (lane 2) cell lysate using PIWIL4 monoclonal antibody (Product # MA5-17151).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PIWIL4 using a PIWIL4 monoclonal antibody (Product # MA5-17151) against a human PIWIL4 recombinant protein.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PIWIL4 using PIWIL4 monoclonal antibody (Product # MA5-17151) in HEK293 (1) and PIWIL4 (AA: 304-434) human IgG Fc transfected HEK293 (2) cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PIWIL4 using PIWIL4 monoclonal antibody (Product # MA5-17151) in PC-3 (1) and PANC-1 (2) cell lysate.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PIWIL4 monoclonal antibody (Product # MA5-17151) with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIWIL4 monoclonal antibody (Product # MA5-17151) with DAB staining

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PIWIL4 monoclonal antibody (Product # MA5-17151) followed with DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIWIL4 monoclonal antibody (Product # MA5-17151) followed with DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using PIWIL4 monoclonal antibody (Product # MA5-17151) (green) and negative control (purple).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of HeLa cells using PIWIL4 monoclonal antibody (Product # MA5-17151) (green) and negative control (red).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 1 Expression of PIWIL genes A1. mRNA expression of PIWIL2 and PIWIL4 in synovial tissues (ST) from RA and OA patients. Mean dCt PIWIL2 in RAST 5.3 and in OAST 5.5; mean dCt PIWIL4 in RAST 3.6 and in OAST 2.9. PIWIL1 and PIWIL3 were not expressed. A2. mRNA expression of PIWIL2 and PIWIL4 in isolated synovial fibroblasts (SF) from RA and OA patients. Mean dCt PIWIL2 in RASF 2.2 and in OASF 1.3; mean dCt PIWIL4 in RASF 1.3 and in OASF 1.2. PIWIL1 and PIWIL3 were not expressed. B. PIWIL4 protein was detected by Western blot in both RASF and OASF in the nucleus, but not in the cytoplasm. PIWIL2 expression was weak. C. Immunofluorescence has shown the presence of PIWIL4 protein predominantly in the cell nucleus and in the perinuclear regions (representative example of a RASF culture).