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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [7]
- Immunohistochemistry [4]
- Flow cytometry [2]
- Other assay [1]
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- Product number
- MA5-17151 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PIWIL4 Monoclonal Antibody (10G9B11)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-17151 targets PIWIL4 in FACS, IHC and WB applications and shows reactivity with Human samples.
- Antibody clone number
- 10G9B11
- Concentration
- 1 mg/mL
Submitted references Expression and Regulation of PIWIL-Proteins and PIWI-Interacting RNAs in Rheumatoid Arthritis.
Pleštilová L, Neidhart M, Russo G, Frank-Bertoncelj M, Ospelt C, Ciurea A, Kolling C, Gay RE, Michel BA, Vencovský J, Gay S, Jüngel A
PloS one 2016;11(11):e0166920
PloS one 2016;11(11):e0166920
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIWIL4 using a PIWIL4 monoclonal antibody (Product # MA5-17151) against a human PIWIL4 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIWIL4 in PC-3 (lane 1) and PANC-1 (lane 2) cell lysate using PIWIL4 monoclonal antibody (Product # MA5-17151).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIWIL4 using PIWIL4 monoclonal antibody (Product # MA5-17151) in HEK293 (1) and PIWIL4 (AA: 304-434) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIWIL4 in PC-3 (lane 1) and PANC-1 (lane 2) cell lysate using PIWIL4 monoclonal antibody (Product # MA5-17151).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIWIL4 using a PIWIL4 monoclonal antibody (Product # MA5-17151) against a human PIWIL4 recombinant protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIWIL4 using PIWIL4 monoclonal antibody (Product # MA5-17151) in HEK293 (1) and PIWIL4 (AA: 304-434) human IgG Fc transfected HEK293 (2) cell lysate.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PIWIL4 using PIWIL4 monoclonal antibody (Product # MA5-17151) in PC-3 (1) and PANC-1 (2) cell lysate.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PIWIL4 monoclonal antibody (Product # MA5-17151) with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIWIL4 monoclonal antibody (Product # MA5-17151) with DAB staining
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using PIWIL4 monoclonal antibody (Product # MA5-17151) followed with DAB staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using PIWIL4 monoclonal antibody (Product # MA5-17151) followed with DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using PIWIL4 monoclonal antibody (Product # MA5-17151) (green) and negative control (purple).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HeLa cells using PIWIL4 monoclonal antibody (Product # MA5-17151) (green) and negative control (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 1 Expression of PIWIL genes A1. mRNA expression of PIWIL2 and PIWIL4 in synovial tissues (ST) from RA and OA patients. Mean dCt PIWIL2 in RAST 5.3 and in OAST 5.5; mean dCt PIWIL4 in RAST 3.6 and in OAST 2.9. PIWIL1 and PIWIL3 were not expressed. A2. mRNA expression of PIWIL2 and PIWIL4 in isolated synovial fibroblasts (SF) from RA and OA patients. Mean dCt PIWIL2 in RASF 2.2 and in OASF 1.3; mean dCt PIWIL4 in RASF 1.3 and in OASF 1.2. PIWIL1 and PIWIL3 were not expressed. B. PIWIL4 protein was detected by Western blot in both RASF and OASF in the nucleus, but not in the cytoplasm. PIWIL2 expression was weak. C. Immunofluorescence has shown the presence of PIWIL4 protein predominantly in the cell nucleus and in the perinuclear regions (representative example of a RASF culture).
