PA5-31456

antibody from Invitrogen Antibodies

Targeting: VPS33A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-31456

Provider

Invitrogen Antibodies

Product name

VPS33A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: 293T, HeLa, HepG2.

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of VPS33A using 30 µg of A549 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a VPS33A polyclonal antibody (Product # PA5-31456) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of VPS33A was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a VPS33A Polyclonal Antibody (Product # PA5-31456) at a dilution of 1:500.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of VPS33A was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS33A Polyclonal Antibody (Product # PA5-31456) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of VPS33A was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: VPS33A Polyclonal Antibody (Product # PA5-31456) diluted at 1:200. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using VPS33A (Product # PA5-31456) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.