Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-94995 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-94995, RRID:AB_1262274
- Product name
- Rabbit Polyclonal ZCCHC8 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references A Two-Layered Targeting Mechanism Underlies Nuclear RNA Sorting by the Human Exosome.
An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression.
Wu G, Schmid M, Rib L, Polak P, Meola N, Sandelin A, Jensen TH
Cell reports 2020 Feb 18;30(7):2387-2401.e5
Cell reports 2020 Feb 18;30(7):2387-2401.e5
An Mtr4/ZFC3H1 complex facilitates turnover of unstable nuclear RNAs to prevent their cytoplasmic transport and global translational repression.
Ogami K, Richard P, Chen Y, Hoque M, Li W, Moresco JJ, Yates JR 3rd, Tian B, Manley JL
Genes & development 2017 Jun 15;31(12):1257-1271
Genes & development 2017 Jun 15;31(12):1257-1271
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: ZCCHC8 Antibody [NB100-94995] - Analysis of ZCCHC8 in HeLa whole cell lsyate using anti-ZCCHC8 antibody. Lane1: Control siRNA , Lane 2: HeLa cells treated with ZCCHC8 siRNA . Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: ZCCHC8 Antibody [NB100-94995] - Detection of Human ZCCHC8 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibody: Affinity purified rabbit anti-ZCCHC8 antibody NB100-94995 used for IP at 3 mcg/mg lysate. ZCCHC8 was also immunoprecipitated by rabbit anti-ZCCHC8 antibody NB100-94996, which recognizes a downstream epitope.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: ZCCHC8 Antibody [NB100-94995] - Sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-used at a dilution of 1:5,000 (0.2ug/ml) and 1:2,000 (0.5ug/ml). Detection: DAB and Red-fluorescent Goat anti-Rabbit IgG-heavy and light chain, cross-adsorbedAntibody DyLight (R) 594 Conjugated used at a dilution of 1:100