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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00005238-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005238-M01, RRID:AB_2161546
- Product name
- Mouse Monoclonal PGM3 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. PGM3 - phosphoglucomutase 3
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi ( Cat # H00005238-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody (M01), clone 1E2-1B12 (Cat # H00005238-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody (M01), clone 1E2-1B12.Lane 1: PGM3 transfected lysate(59.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: PGM3 Antibody (1E2-1B12) [H00005238-M01] - PGM3 monoclonal antibody (M01), clone 1E2-1B12 Analysis of PGM3 expression in HeLa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Detection limit for recombinant GST tagged PGM3 is approximately 3ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of PGM3 transfected lysate using anti-PGM3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM3 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: PGM3 Antibody (1E2-1B12) [H00005238-M01] - Analysis of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. Antibody concentration 2 ug/ml.
