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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [6]
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- Validations
- Western blot [1]
- Immunoprecipitation [2]
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- Product number
- NB100-57542 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-57542, RRID:AB_2186216
- Product name
- Rabbit Polyclonal Senataxin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Dual Processing of R-Loops and Topoisomerase I Induces Transcription-Dependent DNA Double-Strand Breaks.
A senataxin-associated exonuclease SAN1 is required for resistance to DNA interstrand cross-links.
Senataxin resolves RNA:DNA hybrids forming at DNA double-strand breaks to prevent translocations.
Sae2/CtIP prevents R-loop accumulation in eukaryotic cells.
Functions of Replication Protein A as a Sensor of R Loops and a Regulator of RNaseH1.
A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage.
Cristini A, Ricci G, Britton S, Salimbeni S, Huang SN, Marinello J, Calsou P, Pommier Y, Favre G, Capranico G, Gromak N, Sordet O
Cell reports 2019 Sep 17;28(12):3167-3181.e6
Cell reports 2019 Sep 17;28(12):3167-3181.e6
A senataxin-associated exonuclease SAN1 is required for resistance to DNA interstrand cross-links.
Andrews AM, McCartney HJ, Errington TM, D'Andrea AD, Macara IG
Nature communications 2018 Jul 3;9(1):2592
Nature communications 2018 Jul 3;9(1):2592
Senataxin resolves RNA:DNA hybrids forming at DNA double-strand breaks to prevent translocations.
Cohen S, Puget N, Lin YL, Clouaire T, Aguirrebengoa M, Rocher V, Pasero P, Canitrot Y, Legube G
Nature communications 2018 Feb 7;9(1):533
Nature communications 2018 Feb 7;9(1):533
Sae2/CtIP prevents R-loop accumulation in eukaryotic cells.
Makharashvili N, Arora S, Yin Y, Fu Q, Wen X, Lee JH, Kao CH, Leung JW, Miller KM, Paull TT
eLife 2018 Dec 7;7
eLife 2018 Dec 7;7
Functions of Replication Protein A as a Sensor of R Loops and a Regulator of RNaseH1.
Nguyen HD, Yadav T, Giri S, Saez B, Graubert TA, Zou L
Molecular cell 2017 Mar 2;65(5):832-847.e4
Molecular cell 2017 Mar 2;65(5):832-847.e4
A SUMO-dependent interaction between Senataxin and the exosome, disrupted in the neurodegenerative disease AOA2, targets the exosome to sites of transcription-induced DNA damage.
Richard P, Feng S, Manley JL
Genes & development 2013 Oct 15;27(20):2227-32
Genes & development 2013 Oct 15;27(20):2227-32
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Senataxin Antibody [NB100-57542] - Whole cell lysate (5 ug) from HeLa, 293T, Jurkat, and A549 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Senataxin antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 75 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Senataxin Antibody [NB100-57542] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Senataxin antibody used for IP at 6 ug per reaction. Senataxin was also immunoprecipitated by a previous lot of this antibody and rabbit anti-Senataxin antibody. For blotting immunoprecipitated Senataxin, the antibody was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: Senataxin Antibody [NB100-57542] - Detection of human Senataxin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Senataxin antibody NB100-57542 used for IP at 3 ug per reaction. Senataxin was also immunoprecipitated by rabbit anti-Senataxin antibody NB100-57543. For blotting immunoprecipitated Senataxin, NB100-57542 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
