Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- PAB10081 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10081, RRID:AB_1679627
- Product name
- ING3 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of ING3.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Phylogenetic analysis of the ING family of PHD finger proteins.
A novel PHD-finger motif protein, p47ING3, modulates p53-mediated transcription, cell cycle control, and apoptosis.
Different HATS of the ING1 gene family.
He GH, Helbing CC, Wagner MJ, Sensen CW, Riabowol K
Molecular biology and evolution 2005 Jan;22(1):104-16
Molecular biology and evolution 2005 Jan;22(1):104-16
A novel PHD-finger motif protein, p47ING3, modulates p53-mediated transcription, cell cycle control, and apoptosis.
Nagashima M, Shiseki M, Pedeux RM, Okamura S, Kitahama-Shiseki M, Miura K, Yokota J, Harris CC
Oncogene 2003 Jan 23;22(3):343-50
Oncogene 2003 Jan 23;22(3):343-50
Different HATS of the ING1 gene family.
Feng X, Hara Y, Riabowol K
Trends in cell biology 2002 Nov;12(11):532-8
Trends in cell biology 2002 Nov;12(11):532-8
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using ING3 polyclonal antibody (Cat # PAB10081) shows detection of a band at ~55 KDa corresponding to ING3 in RKO cells transfected with ING3 (Lane 2).Control RKO cells do not show detection of this specific band (lane1).The identity of the non-specific bands at 33 KDa and 20 KDa has not been determined.Each lane contains approximately 10 ug of RKO whole cell lysate (ATCC# CRL-2577 - human coloncancer) separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose.After blocking with 5% NF dry milk, the membranewas probed with the primary antibody diluted to1:1,000.Incubation was at 4°C overnight followed by washes and reaction with a 1:20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG[H&L] MXHu for 45 min at room temperature.IRDye™800 fluorescence imagewas captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye isa trademark of LI-COR, Inc.
