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Learn600-101-283
antibody from Rockland Immunochemicals, Inc.
Targeting: ING3
Eaf4, FLJ20089, MEAF4, p47ING3
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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- Product number
- 600-101-283 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-101-283, RRID:AB_2233928
- Product name
- Anti-p47 ING3 (GOAT) Antibody - 600-101-283
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
- Submitted by
- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western blot using Rockland's purified anti-ING3 antibody shows detection of a band at ~55 kDa corresponding to ING3 in RKO cells transfected with ING3 (lane 2). Control RKO cells do not show detection of this specific band (lane 1). The identity of the non-specific bands at 33 kDa and 20 kDa has not been determined. Each lane contains approximately 10 µg of RKO whole cell lysate (ATCC# CRL-2577 - human colon cancer) separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred to nitrocellulose. After blocking with 5% NF dry milk, the membrane was probed with the primary antibody diluted to 1:1,000. Incubation was at 4° C overnight followed by washes and reaction with a 1:20,000 dilution of IRDye800 conjugated Rb-a-Goat IgG [H&L] MXHu (605-432-013) for 45 min at room temperature. IRDye800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
- Validation comment
- Western Blot
