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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 60282-1-IG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FAM3C Monoclonal Antibody (2E10D5)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Immunogen sequence: KMDASLGNL FARSALDTAA RSTKPPRYKC GISKACPEKH FAFKMASGAA NVVGPKICLE DNVLMSGVKN NVGRGINVAL ANGKTGEVLD TKYFDMWGGD VAPFIEFLKA IQDGTIVLMG TYDDGATKLN DEARRLIADL GSTSITNLGF RDNWVFCGGK GIKTKSPFEQ HIKNNKDTNK YEGWPEVVEM EGCIPQKQD (30-227 aa encoded by BC046932)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 2E10D5
- Vial size
- 150 µL
- Concentration
- 0.62 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fetal human brain tissue were subjected to SDS PAGE followed by western blot with 60282-1-IG ( FAM3C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed HepG2 cells using 60282-1-IG (FAM3C antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-IG ( FAM3C Antibody) at dilution of 1:200 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human oesophagus cancer tissue slide using 60282-1-IG ( FAM3C Antibody) at dilution of 1:200 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).
