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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Other assay [1]
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Validation data
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- Product number
- 25009-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZC3H12A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: ANALLSPPR APSKDKNGRR PSPSSQSSSL LTESEQCSLD GKKLGAQASP GSRQEGLTQT YAPSGRSLAP SGGSGSSFGP TDWLPQTLDS LPYVSQDCLD SGIGSLESQM SELWGVRGGG PGEPGPPRAP YTGYSPYGSE LPATAAFSAF GRAMGAGHFS VPADYPPAPP AFPPREYWSE PYPLPPPTSV LQEPPVQSPG AGRSPWGRAD SLAKEQASVY TKLCGVFPPH LVEAVMGRFP QLLDPQQLAA EILSYKSQHP SE (339-599 aa encoded by BC005001)
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.48 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Raji cells were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- K-562 cells were subjected to SDS PAGE followed by western blot with 25009-1-AP (ZC3H12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 25009-1-AP (ZC3H12A Antibody) at dilution of 1:50 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human small intestine tissue slide using 25009-1-AP ( ZC3H12A Antibody) at dilution of 1:50 (under 40x lens).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-ZC3H12A (IP:25009-1-AP, 4ug; Detection:25009-1-AP 1:300) with Raji cells lysate 2000ug.
