- PA5-22137 - Invitrogen Antibodies ZC3H12A antibody

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western blot analysis of ZC3H12A using Various whole cell extracts (30 µg). Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a ZC3H12A polyclonal antibody (Product # PA5-22137) at a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot analysis of ZC3H12A was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a ZC3H12A Polyclonal Antibody (Product # PA5-22137) at a dilution of 1:1000 and a HRP-conjugated anti-rabbit IgG secondary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-ZC3H12A Polyclonal Antibody (Product # PA5-22137) and a ~65 kDa band corresponding to Endoribonuclease ZC3H12A was observed across cell lines and tissues tested . Whole cell extracts (30 µg lysate) of THP-1 (Lane 1), THP-1 treated with 1 µg/mL LPS for 16h (Lane 2), SK-O-V3 (Lane 3), Raji (Lane 4), SH-SY5Y (Lane 5), IMR-32 (Lane 6), Mouse Heart (Lane 7), were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036,1:20000 using the iBright™ FL1500 Imaging System (Product # A44115). Chemiluminescent detection was performed using SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Product # 34580).Signal was induced in THP-1 cells treated with LPS as seen in lanes 1 and 2.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using ZC3H12A Polyclonal Antibody (Product # PA5-22137). Untreated (–) and treated (+) THP-1 whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ZC3H12A Polyclonal Antibody (Product # PA5-22137) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockdown of Endoribonuclease ZC3H12A was achieved by transfecting Raji with Endoribonuclease ZC3H12A specific siRNAs (Silencer® select Product # s36970, s36971). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Endoribonuclease ZC3H12A knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with ZC3H12A Polyclonal Antibody (Product # PA5-22137, 1:1000 ) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:20000). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Endoribonuclease ZC3H12A.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescent analysis of ZC3H12A in paraformaldehyde-fixed HeLa cells using a ZC3H12A polyclonal antibody (Product # PA5-22137) at a 1:200 dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: ZC3H12A antibody detects ZC3H12A protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: ZC3H12A stained by ZC3H12A antibody (Product # PA5-22137) diluted at 1:500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunohistochemistry (Paraffin) analysis of ZC3H12A was performed in paraffin-embedded mouse kidney tissue using ZC3H12A Polyclonal Antibody (Product # PA5-22137) at a dilution of 1:250.

PA5-22137