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- Antibody Data
- Antigen structure
- References [4]
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- Validations
- Western blot [1]
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- Product number
- MAB7875 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse MCPIP1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human MCPIP1 in direct ELISAs and human and mouse MCPIP1 in Western blots. In direct ELISAs, no cross-reactivity with recombinant human MCPIP3 is observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q5D1E8- Isotype
- IgG
- Antibody clone number
- 604421
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Bcl10-controlled Malt1 paracaspase activity is key for the immune suppressive function of regulatory T cells.
Autocleavage of the paracaspase MALT1 at Arg-781 attenuates NF-κB signaling and regulates the growth of activated B-cell like diffuse large B-cell lymphoma cells.
Malt1 self-cleavage is critical for regulatory T cell homeostasis and anti-tumor immunity in mice.
Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation.
Rosenbaum M, Gewies A, Pechloff K, Heuser C, Engleitner T, Gehring T, Hartjes L, Krebs S, Krappmann D, Kriegsmann M, Weichert W, Rad R, Kurts C, Ruland J
Nature communications 2019 May 28;10(1):2352
Nature communications 2019 May 28;10(1):2352
Autocleavage of the paracaspase MALT1 at Arg-781 attenuates NF-κB signaling and regulates the growth of activated B-cell like diffuse large B-cell lymphoma cells.
Wu CH, Yang YH, Chen MR, Tsai CH, Cheng AL, Doong SL
PloS one 2018;13(6):e0199779
PloS one 2018;13(6):e0199779
Malt1 self-cleavage is critical for regulatory T cell homeostasis and anti-tumor immunity in mice.
Baens M, Stirparo R, Lampi Y, Verbeke D, Vandepoel R, Cools J, Marynen P, de Bock CE, Bornschein S
European journal of immunology 2018 Oct;48(10):1728-1738
European journal of immunology 2018 Oct;48(10):1728-1738
Aggregation of Human Trophoblast Cells into Three-Dimensional Culture System Enhances Anti-Inflammatory Characteristics through Cytoskeleton Regulation.
Seno K, Munakata Y, Sano M, Kawahara-Miki R, Takahashi H, Ohkuchi A, Iwata H, Kuwayama T, Shirasuna K
International journal of molecular sciences 2018 Aug 8;19(8)
International journal of molecular sciences 2018 Aug 8;19(8)
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Mouse MCPIP1 by Western Blot. Western blot shows lysates of untreated (-) HeLa human cervical epithelial carcinoma cell line and RAW 264.7 mouse monocyte/macrophage cell line and THP-1 human acute monocytic leukemia cell line untreated (-) or treated (+) with 1 µg/mL LPS for 24 hours or 10 µg/mL LPS for 3 hours, respectively. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human MCPIP1 Monoclonal Antibody (Catalog # MAB7875) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for MCPIP1 at approximately 70 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
