Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunoprecipitation [1]
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Validation data
Reference
Comment
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- Product number
- H00253935-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00253935-M01, RRID:AB_518654
- Product name
- ANGPTL5 monoclonal antibody (M01), clone 1B2
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a full length recombinant ANGPTL5.
- Antigen sequence
MMSPSQASLLFLNVCIFICGEAVQGNCVHHSTDSS
VVNIVEDGSNAKDESKSNDTVCKEDCEESCDVKTK
ITREEKHFMCRNLQNSIVSYTRSTKKLLRNMMDEQ
QASLDYLSNQVNELMNRVLLLTTEVFRKQLDPFPH
RPVQSHGLDCTDIKDTIGSVTKTPSGLYIIHPEGS
SYPFEVMCDMDYRGGGWTVIQKRIDGIIDFQRLWC
DYLDGFGDLLGEFWLGLKKIFYIVNQKNTSFMLYV
ALESEDDTLAYASYDNFWLEDETRFFKMHLGRYSG
NAGDAFRGLKREDNQNAKPFSTSDVDNDGCRPACL
VNGQSVKSCSHLHNKTGWWFNECGLANLNGIHHFS
GKLLATGIQWGTWTKNNSPVKIKSVSMKIRRMYNP
YFK- Isotype
- IgG
- Antibody clone number
- 1B2
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of ANGPTL5 expression in transfected 293T cell line by ANGPTL5 monoclonal antibody (M01), clone 1B2.Lane 1: ANGPTL5 transfected lysate(44.2 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ANGPTL5 monoclonal antibody (M01), clone 1B2. Western Blot analysis of ANGPTL5 expression in human kidney.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged ANGPTL5 is 10 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of ANGPTL5 transfected lysate using anti-ANGPTL5 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with ANGPTL5 MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol