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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- GTX113860 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113860, RRID:AB_10620655
- Product name
- UNG antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Initiation of the ATM-Chk2 DNA damage response through the base excision repair pathway.
Chou WC, Hu LY, Hsiung CN, Shen CY
Carcinogenesis 2015 Aug;36(8):832-40
Carcinogenesis 2015 Aug;36(8):832-40
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- UNG antibody detects UNG protein by Western blot analysis.A. 30 ?g HeLa whole cell lysate/extract B. 30 ?g whole cell lysate/extract of UNG shRNA#1-transfected HeLa cellsC. 30 ug whole cell lysate/extract of UNG shRNA#2-transfected HeLa cells11 % SDS-PAGEUNG antibody (GTX113860) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- UNG antibody detects UNG protein by Western blot analysis.A. 30 ?g HeLa whole cell lysate/extract B. 30 ?g whole cell lysate/extract of UNG shRNA#1-transfected HeLa cellsC. 30 ug whole cell lysate/extract of UNG shRNA#2-transfected HeLa cells11 % SDS-PAGEUNG antibody (GTX113860) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- UNG antibody detects UNG protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: UNG protein stained by UNG antibody (GTX113860) diluted at 1:500.Blue: Hoechst 33342 staining.
