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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- PA5-20265 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- UNG Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is C2C12 cell lysate.
- Concentration
- 1 mg/mL
Submitted references An Apoptotic Gene Signature for the Prognosis of Hepatocellular Carcinoma.
Chen K, Zhu P, Liao Y, Yan L, Feng R, Zhai W
OncoTargets and therapy 2021;14:1589-1604
OncoTargets and therapy 2021;14:1589-1604
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of C2C12 cell lysate using a UNG1 polyclonal antibody (Product # PA5-20265) at (A) 0.5, (B) 1 and (C) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Mouse C2C12 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: UNG Polyclonal Antibody (Product # PA5-20265) (A: 0.5 µg/mL, B: 1 µg/mL and C: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed human heart labeling UNG1 with UNG Polyclonal Antibody (Product # PA5-20265) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human heart tissue using UNG Polyclonal Antibody (Product # PA5-20265) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Extra validation in the test set. ( A ) Relative mRNA expression of SOX4, UNG, NRP1, IL2RB and IVNS1ABP in 7 HCC and paired normal tissues. ( B ) Representative Western blot images of the five proteins. ( C ) The Western blot results are normalized to GAPDH. ( D ) Survival analysis in the test set. *P
