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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- AP13924PU-N - Provider product page
- Provider
- OriGene
- Product name
- SPHK2 (N-term) rabbit polyclonal antibody, Purified
- Antibody type
- Polyclonal
- Description
- SPHK2 (N-term) rabbit polyclonal antibody, Purified
- Host
- Rabbit
- Conjugate
- Unconjugated
- Epitope
- SPHK2
- Antibody clone number
- NULL
- Vial size
- 400 µl
- Concentration
- lot specific
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- The anti-SphK2 Pab is used in Western blot (Lane 1) to detect c-myc-tagged SphK2 in transfected 293 cell lysate (a c-myc antibody is used as control in Lane 2). Data is kindly provided by Dr. J. Van Brocklyn from the Ohio State University (Columbus, OH).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human cancer tissue (hepatocarcinoma) reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- IHC
