MA5-31906

antibody from Invitrogen Antibodies

Targeting: SPHK2

ELISA (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-31906

Provider

Invitrogen Antibodies

Product name

SPHK2 Monoclonal Antibody (9C5E1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

MA5-31906 has been tested in indirect ELISA.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

9C5E1

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

ELISA

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of SPHK2 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with SPHK2 monoclonal antibody (Product # MA5-31906) using a dilution of 1:10,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

ELISA analysis of SPHK2 in Control Antigen (black line, 100 ng); Antigen (purple line, 10 ng); Antigen (blue line, 50 ng); Antigen (red line, 100 ng). Samples were incubated with SPHK2 monoclonal antibody (Product # MA5-31906) using a dilution of 1:10,000.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SPHK2 in HeLa cells (green). Sample was incubated with SPHK2 monoclonal antibody (Product # MA5-31906) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry analysis of SPHK2 in HeLa cells (green). Sample was incubated with SPHK2 monoclonal antibody (Product # MA5-31906) using a dilution of 1:200-1:1000 followed by DRAQ5 fluorescent DNA dye (blue), and Alexa Fluor- 555 phalloidin (red labeled actin filaments) .

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SPHK2 in paraffin-embedded endometrial cancer tissue. Sample was incubated with SPHK2 monoclonal antibody (Product # MA5-31906) using a dilution of 1:200-1:1000 followed by DAB staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of SPHK2 in paraffin-embedded bladder cancer tissue. Sample was incubated with SPHK2 monoclonal antibody (Product # MA5-31906) using a dilution of 1:200-1:1000 followed by DAB staining.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of SPHK2 in HeLa cells (green). Sample was incubated with SPHK2 monoclonal antibody (Product # MA5-31906) using a dilution of 1:200-1:400 followed by negative control (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of SPHK2 in HeLa cells (green). Sample was incubated with SPHK2 monoclonal antibody (Product # MA5-31906) using a dilution of 1:200-1:400 followed by negative control (red).