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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [5]
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- Validations
- Immunohistochemistry [1]
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- Product number
- AF3474 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse R-Spondin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse R-Spondin 1 in direct ELISAs and Western blots. In direct ELISAs, approximately 60% cross-reactivity with recombinant human R-Spondin 1 is observed and less than 5% cross-reactivity with recombinant mouse (rm) R-Spondin 3 and rmR-Spondin 4 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9Z132- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references RSPOs facilitated HSC activation and promoted hepatic fibrogenesis.
R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis.
Bone marrow stromal cell transplantation mitigates radiation-induced gastrointestinal syndrome in mice.
R-spondin1 deficiency in mice improves glycaemic control in association with increased beta cell mass.
Human RSPO1/R-spondin1 is expressed during early ovary development and augments β-catenin signaling.
Yin X, Yi H, Wang L, Wu W, Wu X, Yu L
Oncotarget 2016 Sep 27;7(39):63767-63778
Oncotarget 2016 Sep 27;7(39):63767-63778
R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis.
Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G
PloS one 2015;10(4):e0124213
PloS one 2015;10(4):e0124213
Bone marrow stromal cell transplantation mitigates radiation-induced gastrointestinal syndrome in mice.
Saha S, Bhanja P, Kabarriti R, Liu L, Alfieri AA, Guha C
PloS one 2011;6(9):e24072
PloS one 2011;6(9):e24072
R-spondin1 deficiency in mice improves glycaemic control in association with increased beta cell mass.
Wong VS, Oh AH, Chassot AA, Chaboissier MC, Brubaker PL
Diabetologia 2011 Jul;54(7):1726-34
Diabetologia 2011 Jul;54(7):1726-34
Human RSPO1/R-spondin1 is expressed during early ovary development and augments β-catenin signaling.
Tomaselli S, Megiorni F, Lin L, Mazzilli MC, Gerrelli D, Majore S, Grammatico P, Achermann JC
PloS one 2011 Jan 28;6(1):e16366
PloS one 2011 Jan 28;6(1):e16366
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- R-Spondin 1 in Mouse Ovary. R-Spondin 1 was detected in immersion fixed frozen sections of E13.5 mouse ovary using Goat Anti-Mouse R-Spondin 1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3474) at 10 µg/mL overnight at 4 °C. Tissue was stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.
