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- References [1]
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- Immunohistochemistry [3]
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- Product number
- NBP1-77354 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-77354, RRID:AB_11030269
- Product name
- Rabbit Polyclonal R-Spondin 1 Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified. At least three isoforms of RSPO1 are known to exist
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references A Refined Culture System for Human Induced Pluripotent Stem Cell-Derived Intestinal Epithelial Organoids.
Takahashi Y, Sato S, Kurashima Y, Yamamoto T, Kurokawa S, Yuki Y, Takemura N, Uematsu S, Lai CY, Otsu M, Matsuno H, Osawa H, Mizushima T, Nishimura J, Hayashi M, Yamaguchi T, Kiyono H
Stem cell reports 2018 Jan 9;10(1):314-328
Stem cell reports 2018 Jan 9;10(1):314-328
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Analysis in human heart tissue lysate with antibody at (A) 1 and (B) 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 15 ug of spleen lysates per lane. Antibodies: RSPO1, 5171 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 30 ng of human RSPO1 recombinant protein per lane. Antibodies: RSPO1, 5171 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: R-Spondin 1 Antibody [NBP1-77354] - Loading: 30 ng of lysates per lane. Lane 1: 0.5ug/ml, Lane 2: 1ug/ml
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: R-Spondin 1 Antibody [NBP1-77354] - Human spleen tissue with RSPO1 antibody at 2.5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: R-Spondin 1 Antibody [NBP1-77354] - Analysis of RSPO1 in human heart tissue with RSPO1 antibody at 2.5 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: R-Spondin 1 Antibody [NBP1-77354] - RSPO1 in human spleen tissue with RSPO1 antibody at 5 ug/ml.
