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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 600-401-C65 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#600-401-C65, RRID:AB_11179926
- Product name
- Anti-POLB (DNA polymerase beta) (RABBIT) Antibody - 600-401-C65
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit Anti-POLß (DNA polymerase beta) Antibody (600-401-C65). Lane 1: LN428 FLAG POLß (control). Lane 2: A172. Load: 35 µg per lane. Primary antibody: POLß antibody at 1:2000 for overnight at 4°C. Secondary antibody: IRDye800 goat anti-rabbit at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~40 kDa, ~40 kDa. Other band(s): unknown band ~75 kDd.
- Validation comment
- Western Blot
