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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PAB3096 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB3096, RRID:AB_1572458
- Product name
- CDCA8 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of CDCA8.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Extensive crosstalk between O-GlcNAcylation and phosphorylation regulates cytokinesis.
A survivin-ran complex regulates spindle formation in tumor cells.
Wang Z, Udeshi ND, Slawson C, Compton PD, Sakabe K, Cheung WD, Shabanowitz J, Hunt DF, Hart GW
Science signaling 2010 Jan 12;3(104):ra2
Science signaling 2010 Jan 12;3(104):ra2
A survivin-ran complex regulates spindle formation in tumor cells.
Xia F, Canovas PM, Guadagno TM, Altieri DC
Molecular and cellular biology 2008 Sep;28(17):5299-311
Molecular and cellular biology 2008 Sep;28(17):5299-311
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of CDCA8 polyclonal antibody (Cat # PAB3096) in HL-60 cell line lysates (35 ug/lane). CDCA8 (arrow) was detected using the purified polyclonal antibody.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with CDCA8 polyclonal antibody (Cat # PAB3096) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Flow cytometric analysis of HL-60 cells using CDCA8 polyclonal antibody (Cat # PAB3096)(bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
- Validation comment
- Flow Cytometry
