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- Antibody Data
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- References [1]
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- Western blot [1]
- Immunohistochemistry [7]
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- Product number
- NBP1-89951 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-89951, RRID:AB_11039168
- Product name
- Rabbit Polyclonal Borealin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human Borealin antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references PRMT6-mediated H3R2me2a guides Aurora B to chromosome arms for proper chromosome segregation.
Kim S, Kim NH, Park JE, Hwang JW, Myung N, Hwang KT, Kim YA, Jang CY, Kim YK
Nature communications 2020 Jan 30;11(1):612
Nature communications 2020 Jan 30;11(1):612
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Borealin Antibody [NBP1-89951] - Analysis in control (vector only transfected HEK293T lysate) and CDCA8 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human testis shows high expression.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human endometrium shows low expression as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human duodenum shows strong nuclear positivity in a subset of glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human lymphoid tissues shows moderate nuclear positivity in germinal center cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Borealin Antibody [NBP1-89951] - Staining in human testis and skeletal muscle tissues using NBP1-89951 antibody. Corresponding CDCA8 RNA-seq data are presented for the same tissues.
