H00005900-D01P

antibody from Abnova Corporation

Targeting: RALGDS RalGEF, RGDS, RGF

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00005900-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00005900-D01P, RRID:AB_1579545

Product name

RALGDS purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human RALGDS protein.

Antigen sequence

MVQRMWAEAAGPAGGAEPLFPGSRRSRSVWDAVRL
EVGVPDSCPVVLHSFTQLDPDLPRPESSTQEIGEE
LINGVIYSISLRKVQLHHGGNKGQRWLGYENESAL
NLYETCKVRTVKAGTLEKLVEHLVPAFQGSDLSYV
TIFLCTYRAFTTTQQVLDLLFKRYGCILPYSDEDG
GPQDQLKNAISSILGTWLDQYSEDFCQPPDFPCLK
QLVAYVQLNMPGSDLERRAHLLLAQLEHSEPIEAE
PEALSPVPALKPTPELELALTPARAPSPVPAPAPE
PEPAPTPAPGSELEVAPAPAPELQQAPEPAVGLES
APAPALELEPAPEQDPAPSQTLELEPAPAPVPSLQ
PSWPSPVVAENGLSEEKPHLLVFPPDLVAEQFTLM
DAELFKKVVPYHCLGSIWSQRDKKGKEHLAPTIRA
TVTQFNSVANCVITTCLGNRSTKAPDRARVVEHWI
EVARECRILKNFSSLYAILSALQSNSIHRLKKTWE
DVSRDSFRIFQKLSEIFSDENNYSLSRELLIKEGT
SKFATLEMNPKRAQKRPKETGIIQGTVPYLGTFLT
DLVMLDTAMKDYLYGRLINFEKRRKEFEVIAQIKL
LQSACNNYSIAPDEQFGAWFRAVERLSETESYNLS
CELEPPSESASNTLRTKKNTAIVKRWSDRQAPSTE
LSTSGSSHSKSCDQLRCGPYLSSGDIADALSVHSA
GSSSSDVEEINISFVPESPDGQEKKFWESASQSSP
ETSGISSASSSTSSSSASTTPVAATRTHKRSVSGL
CNSSSALPLYNQQVGDCCIIRVSLDVDNGNMYKSI
LVTSQDKAPAVIRKAMDKHNLEEEEPEDYELLQIL
SDDRKLKIPENANVFYAMNSTANYDFVLKKRTFTK
GVKVKHGASSTLPRMKQKGLKIAKGIF


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of RALGDS expression in transfected 293T cell line (H00005900-T01) by RALGDS MaxPab polyclonal antibody.Lane 1: RALGDS transfected lysate(99.30 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between RALGDS and HRAS. HeLa cells were stained with anti-RALGDS rabbit purified polyclonal 1:1200 and anti-HRAS mouse purified polyclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)