Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- GTX76675 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX76675, RRID:AB_510219
- Product name
- AS160 / TBC1D4 (phospho Thr642) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Puerarin attenuates palmitate-induced mitochondrial dysfunction, impaired mitophagy and inflammation in L6 myotubes.
Intermittent fasting does not affect whole-body glucose, lipid, or protein metabolism.
Muscle adaptation to short-term fasting in healthy lean humans.
Chen X, Yi L, Song S, Wang L, Liang Q, Wang Y, Wu Y, Gao Q
Life sciences 2018 Aug 1;206:84-92
Life sciences 2018 Aug 1;206:84-92
Intermittent fasting does not affect whole-body glucose, lipid, or protein metabolism.
Soeters MR, Lammers NM, Dubbelhuis PF, Ackermans M, Jonkers-Schuitema CF, Fliers E, Sauerwein HP, Aerts JM, Serlie MJ
The American journal of clinical nutrition 2009 Nov;90(5):1244-51
The American journal of clinical nutrition 2009 Nov;90(5):1244-51
Muscle adaptation to short-term fasting in healthy lean humans.
Soeters MR, Sauerwein HP, Dubbelhuis PF, Groener JE, Ackermans MT, Fliers E, Aerts JM, Serlie MJ
The Journal of clinical endocrinology and metabolism 2008 Jul;93(7):2900-3
The Journal of clinical endocrinology and metabolism 2008 Jul;93(7):2900-3
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Supportive validation
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- GeneTex (provider)
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- Experimental details
- Lysates prepared from 3T3-L1 cells (1-4) or COS cells over-expressing WT AS160 (5, 6) or mutant AS160 T642A, treated with PI-3-Kinase inhibitor LY294002 (5), insulin (1-4, 6-7), were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF